?　　Foreword　　

In 1993, Belgian scientists discovered a new antibody-nano-antibody (VHHs) in camel serum, which is different from the traditional antibody structure and only consists of heavy chain variable regions. Compared with traditional antibodies, nano-antibodies have the advantages of stable physical and chemical properties, low immunogenicity, strong tissue permeability, and can recognize epitopes that traditional antibodies cannot recognize, which makes them a strong and beneficial supplement to traditional antibodies.

Nano-antibody is only composed of heavy chain variable region, with small molecular weight and simple structure, which enables it to realize stable expression in various expression systems. So far, VHHs has been expressed in many expression systems, from prokaryotic cells, yeast, fungi, insect cells, mammalian cell lines to plants. This paper mainly introduces the expression of nano-antibody in different expression systems, and the present situation and development trend in the field of this system.

Prokaryotic expression system　Prokaryotic expression system is the earliest mature protein expression system studied. Obtaining foreign proteins through prokaryotes can obtain gene expression products in a short time, and the cost required is relatively low. The expression system mainly includes Escherichia coli expression, Bacillus subtilis expression and Streptomyces expression. Among them, Escherichia coli system is the most widely used, and the prokaryotic system generally refers to the expression of Escherichia coli.

Escherichia coli expression system　Escherichia coli expression system is the most important expression platform of recombinant protein in laboratory environment, and the expression is controlled by lactose operon. The host of Escherichia coli system has the advantages of fast growth, simple culture, low price, clear genetic background and high protein expression. However, it can not modify the expression product after translation, and the expression product contains a lot of endotoxin and is easy to form inclusion bodies.

Common host bacteria of Escherichia coli expression system

Periplasmic space, as the only compartment with oxidation environment in Escherichia coli, is the most commonly used expression system of nano-antibodies containing specific disulfide bonds. In addition to aerobic environment, there are many partners and isomerases in the periplasmic space, which can promote the correct formation of disulfide bonds and help protein fold. Nano-antibody protein is first produced in the cytoplasmic space, and then secreted into the periplasmic space through Sec pathway, SPR pathway or Tat pathway. After signal peptide is removed by signal peptidase, the target protein can be obtained [1].

Up to now, many VHHs have been successfully expressed in periplasm, some of which can reach tens of milligrams per liter, but there are still a lot of VHHs with low yield. This is because many fusion proteins can not be effectively secreted in the periplasmic space, and cytoplasmic expression seems to be the only expression mode of such fusion proteins [8].

The cytoplasm is a reduced compartment, so that the proteins expressed through the cytoplasm can not be folded correctly, and most of them are inclusion body proteins. The proteins are protected from the degradation of intracellular enzymes and are easy to be separated in high purity and concentration. However, there are many kinds of proteins in cytoplasm, and it is difficult to purify them. At the same time, nonfunctional inclusion proteins need to be completely denatured and refolded to be recovered from these inclusions, but the recovery of VHHs is usually inefficient [1]. In order to solve the problem of cytoplasmic expression, Xue X and others designed a new dialysis-dilution device in 2016. Using this device, researchers refolded VHHs and achieved a recovery rate of 85%, while the conventional dialysis and dilution methods only reached 13%[9]. Bacterial cytoplasm can be an effective compartment to produce correctly folded nanoparticles when expressed by recombinant vectors with sulfhydryl oxidase and disulfide isomerase activities. Larger volume and wider cytoplasm of chaperone/folding enzyme can achieve high yield of nano-antibody [6]. At the same time, double mutants with defects in thioredoxin and glutathione pathways have been developed. Some of these strains have been sold commercially in recent years, such as Origami (Novagen), Rosetta-gami (Novagen) and SHuffle?T7 (New England Biolabs) [10]. Among them, Shufflet 7 overexpresses a critical periplasmic isomerase DsbC, which can effectively catalyze the isomerization of disulfide bonds. Some studies have found that,The activity of isomerase DsbC can increase the yield of functional recombinant protein in periplasmic research [11].　　

Kavousipour Soudabeh and other modified vectors added four different signal peptide sequences to guide the secretion and expression of nano-antibodies, and found that TrBC signal peptide had the best effect [5]. Jianli Yu et al. used repetitive amino acid sequence motifs (GAGAGS) found in silk fibroin (SFP) as a new fusion tag (SF-tag) to enhance the expression of nanosomes in E.coli.. The transcription level of labeled protein is 2.3-7.0 times that of unlabeled protein, and the yield is increased by 2.5-7.1 times [27]. Xi Xie and others tried to select antifreeze protein AXX, and obtained its reverse transcription protein XXA by synthesizing XXA gene, which was used to develop new soluble fusion tags. It was found that the solubility ratio of fusion protein expressed by XXA fusion tag reached 86%, which was much higher than that of other tags [12].

Other prokaryotic expression systems　Lactic acid bacteria are the most common intestinal probiotics, which are generally considered to be harmless to human body and are very suitable for the production host of therapeutic molecules. However, the existing expression data show that the yield of VHHS produced by lactic acid bacteria is low, about 1-3mg/L, which is much lower than that of Escherichia coli [13,14]. Lactobacillus rhamnosus DSM14870, as a normal flora in human vagina, can express HIV antibody in the form of soluble or cell wall anchor fixation, and is a suitable expression host for HIV antibody [2]. When Bacillus brevis choshinensis was used for 3L fed-batch fermentation, the yield was as high as 3g/L[15]. This system is easy to manipulate genes, has no endotoxin and shows weak protease activity, so it is a very noteworthy system.

Eukaryotic expression system　

Due to the defects of prokaryotic expression system, such as difficult purification of inclusion body protein and incomplete protein modification, people began to use eukaryotic expression system to study genes. At present, eukaryotic expression systems commonly used in genetic engineering research include yeast expression system, insect cell expression system and mammalian cell expression system.

Yeast expression system　Yeast system has clear genetic background, simple operation, easy genetic operation, perfect eukaryotic protein expression control system and advantages of both prokaryotic and eukaryotic expression systems. Yeast eukaryotic protein expression systems include methanol yeast expression system, Saccharomyces cerevisiae expression system, Schizosaccharomyces expression system and Kluyveromyces expression system [17].

Saccharomyces cerevisiae is the first yeast fermented by human beings, which has no specific virus and low cost. At present, it is the largest yeast expression system in shake flask culture, with the output exceeding 100mg/L[16]. Some amino acids in VHHs can participate in the recruitment of molecular chaperones in endoplasmic reticulum, thus narrowing the difference of molecular chaperones between Saccharomyces cerevisiae and mammalian cells, which is beneficial to the folding kinetics of nano-antibodies and improving the yield of nano-antibodies in Saccharomyces cerevisiae [17]. The protein expressed by Saccharomyces cerevisiae is prone to hyperglycosylation, and a large number of endogenous proteins are produced during fermentation, so the secretion efficiency of the target protein is low [1]. Considering the above problems, Saccharomyces cerevisiae is gradually replaced by methanol yeast represented by Pichia pastoris.

Pichia pastoris expression system is an efficient expression system of foreign proteins. This microorganism does not accumulate toxic ethanol, but only secretes a few endogenous proteins, and its glycosylation degree is the same as that of mammalian cells, so its immunogenicity is weak, so it is the most commonly used eukaryotic expression system. Pichia pastoris has a strictly regulated methanol inducible AOX1 promoter, which is suitable for high expression of foreign proteins [1]. But methanol is a highly toxic and dangerous chemical product. The GAP promoter without methanol induction is used instead of AXOI, and the carbon source does not need to be changed during the culture process, so the operation is simpler and the time for foreign proteins to reach the peak level can be shortened. The yield and affinity of nanoparticles produced by this system are similar to those produced by methanol-induced expression. It has been studied that the yield of nano-antibody expressed by this system in shake flask is 51.71mg/L[18] and 16mg/L[19].

Fungal expression system　

Some filamentous fungi, such as Trichoderma and Aspergillus, can secrete a large number of foreign proteins in the culture medium. However, the fungal system not only expresses protein of interest, but also produces many proteases, which affects the yield. Joosten et al. obtained only 1.5 mg/L VHHs in Aspergillus awamori medium. After adding 0.25% BSA, it reached 7 mg/L. It is reported that more than 80% of VHH is degraded or adhered to the cell wall compared with endogenous xylanase secreted efficiently in this system, and researchers believe that the low secretion efficiency of VHHs is the bottleneck of fungal expression [20]. In order to overcome this limitation and protect VHHs from degradation, other researchers fused VHH with the high amylase A signal sequence (sTAA) of Rhizopus oryzae lipase (N28) and the N-terminal 28 amino acid fragment (N28), so that the highest yield of VHHs expressed in Aspergillus oryzae reached 73mg/L, which was 300-800 mg/L lower than that of Escherichia coli [21]. The final yield of GlaB-VHH fusion protein in Aspergillus oryzae can reach 610mg/L, which provides the possibility for high expression of nano-antibody, but its stability needs to be verified [22]. In addition, VHH[23] was expressed by maize head smut in unconventional secretion pathway.

Insect cell expression system　

Insect cells are a widely used eukaryotic expression system, and recombinant baculovirus vectors (such as AcMNPV) can specifically transfect insect cells. Its survival in nature is short, and the strong promoter is inactivated in mammals and cannot infect vertebrates, so the recombinant baculovirus is safe for human beings. Proteins with complex structures under the control of strong promoters may not be able to form correct folding and post-translation modification, which may lead to the aggregation of target proteins and reduce antibody production, so the expression yield of VHHs in this system is much higher than that of other types of antibodies [17].

Gómez-Sebastián and others used the improved baculovirus expression system (IBES? technology) to express VHHs for the first time. The chigger larvae were inoculated with different recombinant baculovirus inoculation methods. The yield of VHH extracted from larvae is 257 mg/L, and the recovery rate after purification is 50%[7]. When expressing anti-endothelial cell selection protein, Drosophila cell SC-2 secreted 12-14mg/L, but the target protein was not detected when expressed in Escherichia coli at the same time, which may be related to the processing and modification ability of insect cells [26].

Mammalian cell expression system　Mammalian cells are the best expression system for therapeutic antibodies, because the produced antibodies have the weakest immunogenicity. At present, the main cell lines include CHO, NS0, Sp2/0, HEK293 and per C6 [17]. Among them, CHO cells grow rapidly and adapt to serum-free suspension culture, which is the most popular host. They can express VHHS up to 100mg/L in shake flask [24]. HEK293 cells are more commonly used in the laboratory because of transient transfection and semi-stable expression, because they require less time and labor. However, monoclonal antibodies approved for the treatment of human diseases are only produced by CHO, NS0 and SP2/0. So far, HEK293 cells have not been used to produce VHHs, and there are few reports about producing antibody fragments by this method [17]. In most literatures, CHO is used to produce antibody fragments. Up to now, 50% of the therapeutic proteins produced in industry are still produced by CHO [1]. The production costs associated with mammalian cell lines are much higher than those associated with any other laboratory research platform.

Common host cells of mammalian expression system

Plant expression system　Compared with other expression systems, plant production platform (stable and transient expression system) is cheap, safe, capable of folding and assembling complex proteins and post-translation modification, easy to expand, and unaffected by human pathogens and diseases. It is an ideal protein expression host and is usually used as an alternative method for mammalian expression systems. In addition, plant expression products can be concentrated in specific organs, such as leaves or seeds, and can be taken orally directly. Tobacco leaves are the most common hosts for expressing VHHs, while the proteins expressed in Arabidopsis and rice seeds have attracted much attention because of their direct oral input of VHHs[1]. The stable transformation methods commonly used in plant system expression have some problems, such as the recombinant gene escaping to nature or the time-consuming transformation. Therefore, some studies have proposed the transient expression system of plant virus expression vector as an alternative method, and the nano-body content with correct conformation reaches 0.45% of the total soluble protein [25].

Present situation and development of nano-antibody expression　

Escherichia coli expression system is the most commonly used expression system of nano-antibody, which has two expression modes: periplasmic expression and cytoplasmic expression, each with its own advantages and disadvantages. Some oxidation engineering strains have been modified, but more experimental support is needed. In addition, various methods of modifying vectors, such as adding labels or signal peptides, have been put forward and have been verified by experiments. Compared with Escherichia coli, Pichia pastoris has excessive glycosylation, which may lead to immunogenicity and ultimately affect its clinical application. The expression systems of fungi, insects, plants and mammals are much more complicated than prokaryotic and eukaryotic expression systems, and are generally not easily adopted after considering the production time, cost and laboratory conditions.

Escherichia coli system is fast, convenient and cheap, and it is the first choice for VHH expression under most laboratory conditions. When some types of VHHs can’t be successfully produced in E.coli, other systems will be used instead.

List of nano-antibody expression

　　Future Network, Beijing, February 7 (Reporter Zhou Xin) At 23: 51 pm on February 6, Xiao ‘an, a girl from Guangzhou who was traveling in Taipei, received an emergency alarm from the earthquake. Ten seconds later, her hotel began to shake, and she and her friends immediately opened the door and rushed downstairs. After gathering with other tenants downstairs for more than half an hour, they chose to go back to their residence because the temperature was too low. Ann and her friends decided to sleep in shifts. However, one night, the earthquake continued, and Xiao An dared not sleep.

　　Xiao ‘an is 22 years old, a senior student and a painter. In early February, during the winter vacation, she started a 9-day and 8-night trip to Taiwan Province with her friends. "Because there were many exhibitions in Taiwan Province before the Chinese New Year, I made an appointment with my friends to come and watch comic-con exhibitions and the like, which just happened to meet Taiwan Province, a classmate I met before."

　　Xiao An and his friends live in a hotel on Xining South Road, Wanhua District, Taipei. Before the earthquake, they had just returned to their residence from the night market in Ximending and were reading a book. She was a little confused when she saw that the mobile phone received the earthquake warning information, but then it shook off guard, which made her and her friends subconsciously run away. "At that time, there was an alarm on the mobile phone about 10 seconds in advance, and we were all confused. Suddenly the building shook and it shook for a long time." Xiao An lives on the sixth floor. When she went downstairs, she noticed that all the tenants were running out at the same time. And the streets are full of people who ran out.

　　In the third minute after the earthquake, Xiao An sent her mother a WeChat, telling her that she had encountered an earthquake. My mother was very worried. She kept asking her what to do, whether she had food or not, and told her to bring water. When my friend’s parents learned the news of the earthquake, they wanted them to change their plane tickets to go home. Xiao An and his friends originally scheduled to go home on the 11th, but before leaving, they thought they could play for one more day, so they changed their air tickets to the 12th. There was an earthquake this time, and Xiao An thought it was impossible to change the visa. "It is estimated that it is unlikely. Now many people want to change their visas."

　　After gathering downstairs for half an hour, the crowd gradually dispersed because of the cold weather. "I can hear dogs barking nearby, and most shops in the street are closing. I went to a convenience store with my friends. Many people were waiting in line to buy food, and everyone was bowing their heads and returning their mobile phone messages. " Xiao An told reporters.

　　Since returning to his residence, Xiao An has been watching the earthquake report on TV. "In fact, there was an earthquake the day before yesterday. At that time, the subway and the train stopped, but there was no feeling in Taipei. We thought it was a small earthquake, and Taiwan Province’s friends also said not to worry. " She told Future.com that she didn’t expect the earthquake to be so serious. "I dare not turn off the TV. I still feel very serious. Several buildings in Hualien are tilted. Although the landlord said that there is no need to be afraid of frequent earthquakes, because the hotel is old and has a wooden structure, it is still very worried. " Ann and her friends decided to sleep in shifts and go down as soon as something happened.

　　At 2 o’clock in the morning on the 7 th, Xiao An told reporters that he felt another obvious aftershock. She began to worry about her trip for the next few days. "I originally planned to go to Jiufen Mountain on the 8 th, and the homestays have been booked. Now I am hesitant and feel unsafe. It may be safer to stay in Taipei."

　　At 3: 16 in the morning, Xiao An told reporters, "It was shocked again, and the shaking was obvious. I am afraid, but I am so sleepy. " Xiao An said that although everyone went back to the room, it was obvious that everyone in the surrounding room was talking and didn’t sleep.

　　According to the China Seismological Network, a 6.5-magnitude earthquake occurred at 23: 50 on February 6 in the waters near Hualien County, Taiwan (24.13 degrees north latitude and 121.71 degrees east longitude) with a focal depth of 11 kilometers. Since then, several earthquakes have occurred at 2: 00, 3: 00 and 4: 00 on the 7 th.

　　According to Xinhua News Agency, as of 5: 00 on the 7th, the Hualien 6.5 earthquake in Taiwan has killed two people and injured 202 others. According to the news of the Taiwan Fire Department at 5 am on the 7th, 149 people have been rescued, and 2 shelters have been opened to accommodate 354 people.

　　In addition to Xiao ‘an and other tourists, many Taiwan Province netizens also told the reporter of Future.com that the earthquake in Taipei, which is more than 100 kilometers away from Hualien, was obvious and swayed from side to side.

　　"I hope that the trip will come back safely now." Xiao An said.

On December 16th, Lectra Auto officially announced that its 08 EM-P car will soon push the Lynk Flyme Auto version 1.7.0 update. This update contains a number of new features and optimizations to enhance the user experience.

The updated contents include: supporting DC charging above -30℃ to enhance the charging ability in low temperature environment; App remote vehicle control function, which is convenient for users to operate vehicles remotely; The heating opening rate of the steering wheel is improved to improve the driving comfort in winter; The map accurately shows the countdown to the next traffic light, which can provide accurate information even in the road without green waves; HUD displays steering tips to enhance driving safety; STR ultra-electric quick start function improves the convenience of vehicle starting; The seat adjustment interface is optimized, from side view to top view, which is more intuitive and easy to understand; Harman Kardon virtual venue returns, and the panoramic sound of WANOS is preserved to enhance the sound experience; Add a cloud listening App to enrich the car entertainment content.

This update is expected to bring a smarter and more convenient driving experience to the owners of Lectra 08 EM-P.

Hualong News (Qi Che) Recently, Kia of yueda announced the launch of EV5 limited-time welfare package. From now until April 30th, consumers can enjoy three years’ financial benefits with 0 down payment and 0 interest when purchasing Kia EV5. In addition, you can also enjoy multiple benefits such as warranty, interconnection, maintenance and replacement of the three power plants and the whole vehicle, with a comprehensive benefit of up to 40,000 yuan.

Specifically, when consumers buy EV5, they can enjoy the financial rights of 0 down payment and 0 interest for 3 years, and the financial discount is as high as 23,000 yuan, which greatly reduces the threshold for consumers to buy a car, which can be described as full of sincerity. In addition, users who purchase EV5 can also enjoy the 8-year or 150,000-kilometer warranty of Sanpower, 5-year or 100,000-kilometer warranty of the whole vehicle, 7,000 yuan replacement subsidy, 5-year free unlimited full data traffic, 5-year free basic maintenance materials, and the opportunity to get a limited number of car purchase rights such as rear cabin air cushions. Not only that, Kia’s old car owners can also get a 2-year compulsory insurance or a 2-year charging card (2,600 yuan /2 years); Consumers can enjoy an additional 5,000 yuan cash subsidy or 5,000 yuan "Happy Purchase" small program coupons donated by yueda Group when buying a car, and the maximum discount can reach 40,000 yuan.

EV5 is a model specially launched by Kia for its 80th anniversary, and it is also the core model of Kia’s electrification transformation. Based on KIA’s world-leading E-GMP platform for electric vehicles, EV5 has a pure and tough appearance design, an efficient three-electric system, a long cruising range of 720km, a flexible and practical ever-changing space, a healthy and environmentally-friendly intelligent cabin, a high-level intelligent driving system and luxurious and comfortable and convenient configuration, bringing users a pure electric travel experience that exceeds expectations.

In terms of the most important three-electric system of pure electric products, EV5 has also shown proud advantages, especially the long-life version of EV5, which has a long pure electric cruising range of 720km, and the charging time from 30% to 80% is only 27 minutes, which can meet the needs of multi-scenarios such as urban commuting or long-distance travel. EV5 also comes standard with a heat pump air conditioning system, a battery temperature control management system, and the only five-level adjustable intelligent kinetic energy recovery system at the same level, which effectively improves power consumption.

In terms of intelligent technology configuration, the EV5 720 is equipped with a 27-inch integrated triple screen, and the long-life version also provides an 11.3-inch head-up display to create an immersive driving experience of "four-screen linkage", and has also cooperated with Baidu and Tencent to develop more than 30 practical apps. In terms of intelligent driving, EV5 has reached L2+ level, and has 18 highly intelligent driving assistance configurations, such as front collision prevention assistance II with active braking and active avoidance, highway driving assistance with automatic lane change by shifting lever, intelligent parking assistance with barrier-free parking in all scenes, collision prevention assistance in blind areas, collision prevention assistance in rear crossing, and safety exit assistance, etc., which can be said to protect safe travel to the extreme.

As a heavy strategic vehicle launched in China and facing the world, Kia EV5 not only carries out R&D and design according to the needs of consumers in China, but also exports to the world with high-quality product value. On April 1st, EV5 export models also began to be mass-produced in Yancheng 4.0 Intelligent Manufacturing Factory and sold to developed markets around the world such as Australia.

　　For users who often travel with the whole family, they will definitely give priority to seven-seat or large six-seat models, but they often find that the third row of many cars is either too small and impractical, or too expensive. Then, you might as well consider it. It will tell you with facts what is really big six-seat and what is comfortable and equal rights.

　　Yipai eπ 008 is the second model launched after the eπ 007 (sedan). It is positioned as a home intelligent large SUV and has two power forms: pure electric and range extension. The body length is more than 5 meters, the wheelbase is more than 3 meters, and it is a really large six-seat, and the internal space is quite generous. Dongfeng Yipai eπ 008 is expected to sell for between 20-250,000 yuan, and has a quite high quality and price ratio among the same-class models. It is expected to be officially delivered in June.

　　Let’s talk about the size of Dongfeng Yipai e π 008. The length, width and height of the body are: 5002 * 1972 * 1732mm, and the wheelbase is 3025mm. You can buy a large SUV of this size at a price 200,000, which is enough to be exciting. The appearance design of Dongfeng Yipai e π 008 is very atmospheric, and the length of 5 meters is quite impressive from the visual experience.

　　In terms of details, the intelligent starlight grille that supports a variety of welcome rhythm display effects, coupled with the through-through headlights composed of 536 LEDs that echo back and forth, is also full of fashion and technology.

　　From the side, the Yi Pai e π 008 adopts a flat roof design, and the area of the C-pillar small window is also quite large, which brings a longer visual length and also gives more vision to the third row, which does not make people feel depressed. The design of the lower waist line adds a little sense of agility to the side of the body, and at the same time, the chrome decorative strips running through the front and rear enhance more luxury. The Yi Pai e π 008 also uses hidden door handles, which makes the body feel stronger and reduces some wind resistance. The wind resistance coefficient of the whole vehicle is 0.268Cd.

　　The EPI 008 uses 21-inch low-drag wheels, which are matched with Continental low-roll-resistance silent tires, with a tire size of 265/45 R21. These four tires alone are worth a lot of money, and it does not cost money. It does greatly improve the comfort and silence of the whole vehicle.

　　The D-pillar side window is equipped with an LED indicator light to display the battery level when charging.

　　The tail design of the Yi Pai e π 008 is relatively full, and the decorative lines picked on both sides of the bumper add some sports elements. The through tail lights and the characteristic lines of the license plate area create a more three-dimensional sense of hierarchy.

　　The interior of the Yipai eπ 008 is a more composed style and is more durable. With a 15.6-inch central LCD screen and an 8.8-inch LCD instrument panel, the car is equipped with an 8155 chip, and the content displayed on the car interface can be seen.

　　The cockpit leather is made of 100% environmentally friendly water-based surface treatment, and the center console is made of Benecke suede PVC material, which feels excellent. The suede material absorbs strong light, and the shadow of the interior does not reflect on the front windshield. This is indeed better than what some luxury brands do.

　　The car-machine interface is not only visible, but also has gesture control functions. In addition, the automatic speech recognition ability of Yipai eπ 008 is very accurate, and it can also determine whether the voice is sent from the front or the back, so as to accurately identify the front and rear functions that need to be controlled.

　　The 9.2-inch streaming rearview mirror shows clearly, and the reflection is very weak. When the rear roof entertainment screen is turned on, the ordinary optical rearview mirror will be blocked by the screen. At this time, the streaming rearview mirror is very necessary, and it can be regarded as a safety configuration.

　　The front 50W mobile phone wireless fast charging, with ventilation function, Honor mobile phone can reach the level of extremely fast charging, basically charging for 1 minute, and the mobile phone power increases by 1%.

　　In addition to buying a large SUV, 200,000 people can also buy a refrigerator, color TV, and a large sofa. The Yipai eπ 008 is equipped with a 15.6-inch rear entertainment screen, which can be opened through the rear touchscreen or voice. For children, the rear entertainment screen can make them quiet faster, and for adults, long-distance travel will not be particularly boring.

　　The upper and lower dual touch screens in the rear row can adjust the heating and ventilation of the seats and the nap mode in the rear row; the touch screens below adjust the cooling and warmth of the refrigerator and move back and forth.

　　Yipai eπ 008 uses a semiconductor refrigeration refrigerator with a minimum temperature of 5 ° C and a maximum temperature of 50 ° C. It has an electric front and rear movement function and can be stopped on the slide at any time, making it convenient for passengers in the second and third rows to pick up drinks and items.　　

　　Of course, Yi Pai e π 008 also has a big sofa – a zero-gravity seat, in addition to supporting ventilation and heating, it also has a massage function, making long-distance travel more comfortable.

　　Yipai eπ 008 benefits from the 3025mm wheelbase, which makes the interior of the vehicle have a large "housing rate" of 67.4%. The direct benefit is that it has 6 large sofa seats, and everyone’s comfort is a mobile home.

　　In terms of details, Yi Pai e π 008’s control is also very good. There are 28 + 2 storage spaces in the whole car, and each row of passengers has its own exclusive cup holder. The trunk space can be flexibly arranged through baffle, which is really attentive.

　　The test drive this time is the Yipai eπ 008 pure electric version, which uses a 200kW rear single motor. From the actual driving experience, it is very easy to drive such a large SUV. The power reserve is good, even if the speed reaches 120km/h, the power can still be stepped on. The steering wheel is pointed more accurately, and the whole car is very flexible and does not feel bulky.

　　The most commendable thing is that the quietness of the whole vehicle is excellent, and it also meets the demands of family car users. With the blessing of Continental silent tires, double front windshield, double front and rear window glass, and the excellent NVH design and materials of the vehicle itself, it is almost impossible to hear obvious fetal noise and wind noise when driving at high speed.

　　Another commendable feature was the suspension tuning. Yi Pai E Pi 008 actually used the coil spring + shock absorber to adjust the feeling of the air spring. The fine road vibration was filtered very thoroughly, and the sharp bounce could be resolved in time.

　　Yipai eπ 008 is based on Dongfeng quantum architecture and is equipped with Dongfeng Mach E electric drive system. The maximum efficiency of the electric drive assembly is 94.5%. The comprehensive energy consumption of the pure electric model is as low as 14.7kwh/100km, and the CLTC battery life reaches 636km. In addition, the extended range model is equipped with the industry’s first extended range dedicated engine with a thermal efficiency of 45.18%. The CLTC pure electric battery life is 210km, the feed fuel consumption is as low as 5.55L/100km, and the CLTC comprehensive battery life is 1300km.

　　Yipai eπ 008 is equipped with 32 high-precision sensing hardware, including a black sesame A1000 chip, which supports high-speed NOA pilot auxiliary driving and has functions such as automatic up and down ramp and automatic lane change, which belongs to the level of L2 + intelligent driving.

　　With the launch of the eπ 007 medium and large sedan and the eπ 008, which will be officially launched in June, Dongfeng Yipai will continue to make efforts in the two power tracks of pure electricity and range extension, and compete head-on with mainstream new energy brands in the 15-250,000 level market. Looking forward to the future Dongfeng Yipai will bring more dazzling products and bring more new energy vehicle choices to consumers.

[Autohome Jinzhong Promotion Channel] Good news! In the Jinzhong area, Toyota is running a high-profile promotion. This high-profile SUV model is now attracting consumers with up to 45,000 discounts, and the minimum selling price has been adjusted to 124,800. This is a rare car purchase opportunity. If you want to know more about specific models and lock in your favorite price, don’t miss it. Click the "Check the car price" button in the quotation form to hurry up and act!

The RAV4 Rongfang is striking with its unique front face design, and the iconic air intake grille adopts the fusion of modernity and power, showing the essence of the Toyota brand. The overall style is stylish and atmospheric, with smooth lines, giving a steady and dynamic visual impact. Whether it is a city shuttle or an outdoor adventure, it can attract everyone’s attention.

The Toyota RAV4 is modern with its sleek body design and streamlined side lines. The body size is 4600mm*1855mm*1680mm and the wheelbase is up to 2690mm, providing a spacious foundation for the interior space. The layout of the front wheelbase of 1605mm and the rear wheelbase of 1620mm guarantees good driving stability. The tire size is 225/65 R17, and it is paired with a stylish wheel design, which is not only practical, but also adds to the sporty atmosphere of the vehicle. Overall, the side design of the RAV4 Rongfang is both beautiful and practical.

The interior design of the RAV4 Rongfang focuses on the coexistence of practicality and comfort, creating a spacious and refined sense of space. The delicate plastic steering wheel feels lightweight and supports manual up, down, and front and rear adjustments, which is convenient for the driver to control accurately. The 10.25-inch central control screen is clear and easy to operate, with integrated multimedia functions. USB and Type-C interfaces are distributed in the front row, which is convenient for passengers to connect to electronic devices. The seats are made of fabric, and the main and auxiliary seats are equipped with multi-directional adjustment functions, including front and rear, backrest and height adjustments, to meet the individual needs of passengers. The second row of seats also supports backrest adjustment, while the rear seats support proportional reclining, providing flexibility for cargo. The overall interior layout is both practical and detail-oriented, providing a pleasant in-car experience for drivers and passengers.

The RAV4 is equipped with a 2.0L displacement four-cylinder engine with a maximum power of 126 kW and a peak torque of 206 Nm. With 171 horsepower output, this engine is coupled with a CVT continuously variable transmission (analog 10th gear), providing the driver with a smooth and efficient power experience.

Summarizing the evaluation of Autohome owners, RAV4 Rongfang left a deep impression with its solid body structure and unique design style. He emphasized the vehicle’s frame skeleton and waist design, as well as the domineering front face, which undoubtedly enhance the driver’s confidence and driving stability. For consumers who pursue individuality and practicality, RAV4 Rongfang is undoubtedly a choice worth considering.

[Autohome Changzhou Promotion Channel] brings you the latest car purchase benefits! In the bustling city of Changzhou, the Mercedes-Benz brand is conducting a powerful promotion. At present, the maximum discount has reached an amazing 111,000 yuan, which brings the starting price of this high-performance luxury SUV down to an attractive 1.0598 million yuan. If you are interested in this passionate and luxurious Mercedes-Benz AMG SUV, don’t miss this once-in-a-lifetime opportunity. Click "Chatti Car Price" in the quotation form to act now and put your dream car within reach at a lower price. Take advantage of this great opportunity to reduce the price and experience the ultimate driving experience brought by AMG!

The Mercedes-Benz GLE coupe AMG presents an unparalleled sporty aesthetic with its unique design language. On the front face, the iconic AMG air intake grille is as sharp as the tusks of a beast, and with the carefully carved front bumper, it gives the vehicle a strong sense of power. The body lines are smooth and powerful, and the back-running design not only enhances the visual impact, but also shows the perfect combination of elegance and dynamics. The overall style is very sporty, and every detail shows the AMG brand’s persistent pursuit of performance and aesthetics.

The AMG Mercedes-Benz GLE coupe is uniquely dynamic and powerful with its streamlined side design. The body length is 4944mm, the width is 2018mm, the height is 1716mm, and the wheelbase is 2935mm, creating a solid body ratio. The smooth and sculptural lines on the side show the AMG brand’s deep understanding of sports aesthetics. The front wheels are equipped with 275/45 R21 tires, and the rear wheels are equipped with wider 315/40 R21 tires, which not only enhances the driving stability, but also adds to the visual impact of the vehicle. The overall design not only focuses on performance, but also pays more attention to the refinement and luxury of details.

The interior design of the Mercedes-Benz GLE Coupe AMG reflects the perfect fusion of luxury and performance. The refined leather material and the steering wheel are wrapped in leather and inlaid with carbon fiber elements, which not only feels delicate to the touch, but also brings a strong sense of movement. The 12.3-inch central control screen stands in front of the driver, clearly displaying multimedia information, navigation and vehicle data, and supports automatic speech recognition for easy operation. The seats, whether in the driver’s or passenger’s seat, are equipped with electric adjustment functions, including front and rear, backrest, high and low and waist support, to ensure ultimate comfort. The front seats also have additional heating and ventilation functions, as well as electric memory functions, which highlight the details of luxury. At the same time, the rear seats support proportional reclining, providing flexible space utilization for passengers and luggage. The overall interior design is both practical and sporty, showcasing the unique interior charm of Mercedes-AMG models.

The Mercedes-Benz GLE Coupe AMG is equipped with a powerful 3.0T turbocharged engine that can output up to 435 horsepower and peak torque of 560 Nm. This power unit is paired with a 9-speed automatic transmission, ensuring excellent performance and driving pleasure.

After summarizing the full text about the excellent performance, unique design and excellent driving experience of the Mercedes-Benz GLE Coupe AMG, we are pleased to learn that in order to give back the support and love of the majority of fans, Mercedes-Benz is now launching a limited-time promotion. In this preferential policy, Mercedes-Benz GLE Coupe AMG is fortunate to enjoy a large market discount, making the dream of owning this high-performance SUV even more within reach. Don’t miss the opportunity, time will never come again. Now is the perfect time for you to experience the ultimate charm of Mercedes-Benz AMG. Seize this opportunity and make Mercedes-Benz GLE Coupe AMG the king’s choice on your road. For more details, please pay attention to Mercedes-Benz official channels or dealers, and ride with speed and passion to ride the future together.

　　China News Service, Hangzhou, May 5th (reporter, Xi Jinyan, Huang Hui) Mo Dao returned late in the spring breeze. This "May Day", "sea of people" has once again become a hot word on the screen. The recovery of the holiday economy means that China’s economy has experienced a steady recovery after the epidemic "stress testing".

　　Taking Zhejiang as a "window" to observe, strolling through major business districts, scenic spots, etc., you will find the reappearance of "one building is hard to find" and "one room is hard to find". Behind it is the mutual force between the supply side and the demand side, and it is also a vivid manifestation of the new development pattern of "double cycle" to release new kinetic energy.

　　New consumption depth empowers double cycle spiral upgrade

　　This holiday, people’s enthusiasm for consumption has warmed up along with the vast land. The reporter observed that during the "May Day" period, new types of consumption such as online shopping, live streaming, consumption of new domestic products, and customized tourism have continued to thrive.

　　In the past few days, the pedestrian street of Xingguang Avenue in Binjiang District of Hangzhou has been crowded, and many time-honored traditional handicrafts have attracted many tourists to stop and admire. Li Jing, director of Zhu’s copper art, told reporters: "This May Day sale is expected to have a historic breakthrough."

　　Li Jing said that on the one hand, because many people responded to the call for the Spring Festival this year, they took this small holiday to "compensate for travel"; on the other hand, people’s consumption concepts are also changing, and the national tide style has led to the revival of time-honored brands.

　　In anticipation of May Day, Mr. Li said they had been "preparing for the war" in the first two months, preparing a variety of incense burners and home furnishings, and temporarily opening offline experience stores in several cities. "I believe there will be a harvest holiday."

　　Under the new development pattern of "double cycle" proposed by the central government, domestic demand has become the main engine driving economic development, and new consumption is a key move to expand domestic demand. Faced with the new trend of consumption, Zhejiang follows the trend and takes the initiative.

　　Sheng Qiuping, director of the Department of Commerce of Zhejiang Province, said that in 2021, Zhejiang will drive new circulation with new infrastructure, give birth to new scenarios, cultivate new models, and promote new consumption. Around the six Zhejiang Li, such as Love Warm Zhejiang Li, Enjoy Zhejiang Li, and Shuizhi Zhejiang Li, more than 1,000 consumption feasts will be created, and various consumption coupons will be issued for more than 1 billion yuan. Continue to serve the people of Zhejiang a consumption feast.

　　As he said, consumption, as the first driver of economic growth, plays an important role in the internal circulation system. In order to seize the opportunity of "May Day", the 2021 National Consumption Promotion Month · Zhejiang Station was launched in Hangzhou before the festival.

　　At the scene, Hangzhou’s first "Digital Intelligence Consumption Carnival", Ningbo "Huixiang Yongcheng" Leading the New Fashion of Consumption ", Huzhou" Thousands of Enterprises Linkage 100 Billion Consumption Thousands of Live Streaming Hosting Thousands of Activities ", Shaoxing" More Huiyue Life, Ancient City Tide Shopping Festival ", Zhoushan" Guochao Zhoushan Hui Aishang New Life "Time-honored Brand Entering Zhoushan and Integrity Demonstration Street Theme Practice Activities, Quzhou Jiangshan" Cloud Life, About’Hui ‘Jiangshan "and other consumer promotion activities were launched simultaneously.

　　Zhao Ying, deputy general manager of Hangzhou Xidebao Group Co., Ltd., a time-honored silk enterprise, introduced that in order to seize the opportunity of holiday consumption, the enterprise actively participated in the consumption promotion month activities and launched fancy promotional activities, in order to achieve the dual development of performance and brand. "We expect the entire’May Day ‘consumption, including e-commerce platforms and offline stores, will usher in new growth."

　　Supply chain collaborative innovation to promote the holiday economy "different red"

　　Chunjiang Plumbing Duck Prophet. The popularity of the "May Day" consumer market is expected for Chen Xinping, a merchant in Yiwu Market who is at the production end.

　　As a global distribution center for small commodities, before the "May Day" holiday, many companies in Yiwu worked overtime and worked at full capacity to catch up with orders, providing production guarantees for domestic and international double circulation.

　　Chen Xinping, head of Meichen Hat Industry, who has been engaged in foreign trade business for 15 years, told reporters that due to the impact of the epidemic, the company tried to do domestic trade last year, and the road to transformation was not smooth sailing. "There are differences in the size, arc and color of hats in the domestic market and foreign markets."

　　After a year of transformation, Chen Xinping said that this year, domestic trade has achieved growth, and foreign trade has taken on many orders transferred from South East Asia. "Now the daily production volume is more than 8,000, and the supply is still in short supply. Orders have been arranged until July."

　　Chen Xinping said that for the "May Day" holiday, the company actively innovated and shipped in advance, incorporating outdoor elements such as beaches and forests into the pattern design of hats, and the market response was relatively good.

　　Zhu Sufang, the head of Yindi Jewelry, is busy delivering goods to "live streaming host" these days. "Many people like to watch live broadcasts on vacation, and the live streaming host has a higher turnover rate." Zhu Sufang told reporters that before "May Day", many live streaming hosts came to "stock up" in advance, and they all started with hundreds or thousands of shipments.

　　From a deeper perspective, the integration of the "double cycle" pattern cannot be separated from the stability of the supply chain system. In recent years, Zhejiang has attached great importance to the modernization of the supply chain, and a number of industrial supply chain collaborative innovation benchmarks have emerged, which continue to input power into the consumer market.

　　For example, Wuchan Zhongda and Zheshang Zhongtuo based on the advantages of commodity collection and supply chain finance, innovated business models, and actively served clusters such as Haining warp knitting and Haiyan fasteners, which effectively promoted the innovation of cluster supply chain models and the reduction of costs and efficiency of enterprises; Zhoushan Ocean Base increased investment in cold chain and commercialized processing facilities, created a whole fishery supply chain from "bow" to "stove", and innovatively launched a squid index and delisting trading system to effectively expand the market radiation range of "Zhoushan seafood".

　　The reporter learned that the Zhejiang Provincial Department of Commerce recently released the "14th Five-Year Plan" for the development of modern supply chains in Zhejiang Province. By 2025, Zhejiang strives to initially build a modern supply chain system for advantageous industries with leading innovation power in the country, efficient coordination of the whole chain, independent, safe and controllable, and cultivate an industrial supply chain system with 3 trillion-level transactions and 10 100-billion-level transactions. As a major open province and a major consumer province, Zhejiang has made overall efforts to fill short boards and forge long boards, providing solid support for building a new development pattern.

　　The flow of things is smooth, and the inner and outer "double circulation" is integrated.

　　The essence of circulation lies in its smooth flow, among which the smooth flow of things is one of the key focuses.

　　During the "May Day" holiday, Ningbo Zhoushan Port was still busy. At 11:18 on May 4, the "Changyi" ship started loading and unloading at berth No. 5 in Meishan Port Area of Ningbo Zhoushan Port. A total of 4,167 TEUs of containers were unloaded here, and 1,615 TEUs were loaded and exported.

　　As a pioneering demonstration area for the construction of smart ports in Ningbo Zhoushan Port, Meishan Port Container Terminal is focusing on piloting smart projects such as remote-controlled bridge cranes, remote-controlled automated tire cranes, and intelligent trucks, with steady improvement in loading and unloading efficiency.

　　"Since the beginning of this year, the operation volume of Ningbo Zhoushan Port Meishan Port has been growing steadily. During the May Day period this year, the market demand for transportation was strong, and ships were concentrated in the port. The average daily container throughput of Meishan Port was more than 20,000 TEUs, a significant increase year-on-year." Mao Hua, the operation manager of Ningbo Meidong Container Terminal Co., Ltd., said.

　　The eyes shifted to the Yiwu railway port, and the freight flow was also endless. During the "May Day" holiday, the "Yixin Europe" China-Europe train shipped 3 to 4 trains every day to ensure the material transportation needs of Eurasian countries and the import needs of the domestic market.

　　"Double circulation" lies in the all-round, wide-ranging, and deep-level interaction and integration of the two domestic and international markets and two resources. As the best adhesive for "double circulation", Zhejiang import enterprises play a good role as bridges and links, vigorously tap the potential of domestic demand, and promote consumption upgrading.

　　Wang Fangmao, head of "Haikelai" imported lifestyle supermarket, told reporters that from the current performance, this year’s "May Day" has increased by 18% compared with the same period last year, especially sunscreen products and imported beverages and other summer products are more popular.

　　It said that this growth is inseparable from an effective market: with the improvement of people’s quality of life, the demand for imported consumer goods is increasing; at the same time, it is also inseparable from a promising government, including the smooth flow of cross-border logistics channels and the organization of various import consumption exhibitions.

　　"Under the global epidemic, ocean freight has risen. We use the’China-Europe Express + Ocean Freight ‘method to combine freight, which is more stable." Wang Fangmao said.

　　Regardless of the wind and waves, it is better to take a stroll. It said that the "May Day" holiday is just a window, and with the continuous advancement of building a new development pattern, the potential of the Chinese market can be expected. (End)

At the "315 party" in Chongqing in 2010, the staff of the 12315 hotline answered complaints from citizens on the spot. Reporter Ouyang Zubing (photo)

Yesterday, the Municipal Administration for Industry and Commerce revealed that this year’s "315 Party" in Chongqing will be broadcast on Chongqing Satellite TV on the evening of March 14th, and five major consumption traps will be exposed in the party. It is reported that Chongqing’s "315 Party" is one of the five major theme activities during this year’s "315" period.

The party will expose five major consumption traps.

This year, the theme of China Consumers Association is "Consumption and People’s Livelihood". Chen Wenyu, deputy director of the Municipal Administration for Industry and Commerce, introduced that this theme was first proposed by the Chongqing Consumer Council and finally adopted, and the theme of this year was determined, with the aim of making all sectors of society fully understand the complementary and mutually promoting relationship between consumption and people’s livelihood. It is reported that the industrial and commercial department of our city will organize a series of activities around this theme during the "315" period this year, including the "315 Party" in Chongqing, which will be broadcast on Chongqing Satellite TV on the evening of March 14th.

Chen Wenzhao introduced that the "315 Party" in Chongqing lasted for 90 minutes. During the party, the industrial and commercial departments will show the achievements of the city’s consumer rights protection work last year through the real picture of law enforcement and case handling, and expose the consumption trap that infringes on the legitimate rights and interests of consumers. There are five typical cases and consumption traps, including "hidden rules of automobile spare tire" and "slimming steel bar", which have attracted consumers’ attention. In addition, the industrial and commercial department of our city will release ten typical cases of consumer rights protection in 2010 to the society on March 13th.

According to the news from the industry and commerce department, in order to cooperate with this year’s "315" activities, in addition to the above-mentioned activities, during the "315" period, the city will also organize "consumer rights protection in action" to crack down on hidden consumption rules in intermediary, commercial advertising, e-commerce, food and other industries to create a safe and secure consumption environment.

Consumer Council opens new complaint channels in Weibo

"From now on, consumers have added a new consumer complaint channel!" Yesterday, Liu Mingsheng, Secretary-General of the Municipal Consumer Council, introduced that the Municipal Consumer Council had recently launched the Consumer Rights Protection Weibo in its official website "Chongqing March 15th Rights Protection Network". The purpose of opening this Weibo is to collect the focus issues that consumers pay attention to and safeguard the legitimate rights and interests of consumers with the help of "Weibo", a new Internet interactive way with convenient, fast and timely information transmission. And through various forms of consumer publicity and education, improve consumers’ awareness of self-protection.

The website of Chongqing March 15th Consumer Rights Protection Weibo is: httP://www.m.cq315.org. In other words, from now on, if citizens encounter consumer fraud, price traps, overlord contracts, etc., they can register online with their real names and then lodge complaints through Weibo. The staff of the Consumer Council will get in touch with you in the shortest time to help you defend your rights.

"The platform of Weibo is actually an interactive platform." Liu Mingsheng said that the Consumer Council will not only arrange special personnel to be on duty, but also add lawyers and industry experts to Weibo to provide correct guidance and suggestions for citizens’ complaints.

At present, the Consumer Council is still improving this Weibo platform. Liu Mingsheng said that before "315" this year, mobile phone users can edit the complaint content and attach the first-hand photo evidence, and send it directly to the Weibo number "315", so that the complaint content can be sent to Weibo. Consumer Council staff will handle it more quickly and conveniently, and rights protection will also shorten the time. At the same time, the Consumer Council formally convened the people’s rights protection enthusiasts in the whole city, and spread the real events about consumption injustice, irregularities, consumption traps and so on around them to Weibo for exposure, so as to jointly safeguard the normal consumption order, protect the rights and interests of consumers, and truly "shout when the road sees injustice".

At the same time, Liu Mingsheng reminded the public that in order to ensure the truthfulness and validity of complaints, citizens who want to make online Weibo complaints must register in real-name registration system. (Reporter Jiang Tao Intern reporter Yan Wei)

related news

Taobao will invest another 200 million anti-counterfeiting consumers this year to view the rights protection process.

Yesterday, Taobao held the 2011 press conference on the Internet’s nationwide rights protection, released the White Paper on Consumer Protection in Taobao in 2010, and announced that on the basis of investing 100 million yuan last year, it will add another 200 million yuan this year to protect consumer rights.

On March 8 last year, Taobao announced that the consumer rights protection platform was officially launched. This is the first consumer rights protection platform initiated by an e-commerce enterprise in China. On the same day, Ma Yun said that in 2010, Taobao will invest 100 million yuan in counterfeiting. Yesterday, Taobao announced that on the basis of this amount, it will invest another 200 million yuan in counterfeiting this year.

At present, Taobao has started the first inquiry responsibility system for online shopping disputes, added a number of rights protection portals on the homepage, transaction details page, community and help center, and launched a 24-hour consumer rights protection platform, so that citizens can see the whole process of rights protection complaints in real time, providing comprehensive protection before, during and after sale.

At present, there are three main ways for Taobao to check whether the seller sells fake goods: machine inspection, Taobao’s commodity standard price library will automatically remove the goods with abnormal prices or no inventory; Manual inspection, a special manual inspection department is set up for 24-hour inspection, and the problem goods are removed at any time; Buyers report that Taobao has a "report this product" button on every product page, and 370 million members on Taobao are commodity inspectors.

Source: Chongqing Business Daily (Reporter Ao Xiangfei Intern Wang Wei) Editor: Feng Yuan Xu Yinjuan

Phoenix Network Technology News (Author/Li Jun) On April 18th, Lei Jun, founder and chairman of Xiaomi, started broadcasting at Xiaomi Auto Delivery Center, and "talked with netizens about the sale of Xiaomi SU7 these days". In the live broadcast, about netizens, "Xiaomi Automobile only took three years to build a car, which is much faster than the industry average speed." This question, Lei Jun responded that Xiaomi has been established for 14 years. Behind Xiaomi Automobile’s 3-year-old car is Xiaomi Group’s 14-year experience and technology accumulation.

Lei Jun said that Xiaomi has 14 years of experience and accumulation, and has set up a very reliable R&D team.Now there are about three or four thousand engineers, of whom at least one thousand are experts. These experts help Xiaomi avoid many detours.Moreover, the industrial chain in China has a good foundation, and it is perfect to be a smart electric vehicle.

In addition, Lei Jun also had a live connection with Wei Jianjun, Chairman of Great Wall Motor. Wei Jianjun said: "Xiaomi has achieved this level in just three years, which is already excellent."