Eating radish in winter and ginger in summer, drinking soup all year round, women love beauty, men love chic, women who drink soup are more beautiful, and men who drink soup are more attractive.

Here in Guangdong, you can find everything you need. There are hundreds of scientifically matched soup packages. You can buy and match all kinds of health-preserving soup materials in one stop. You usually want to drink soup. You don’t need to mix materials, and you don’t have to worry about cooking anything every day. Take it home, wash it and add the main ingredients, and you can enjoy it.

Don’t you feel excited about such a convenient, time-saving and time-saving soup-making material supermarket? Ok, let’s get to the point. Today, I recommend a week’s soup spectrum, which is suitable for all ages. Let’s learn together!

Monday dried cabbage and lily soup

Ingredients: pork show 400g (pork lungs can be changed), carrots, dried vegetables, lilies, apricot, figs and candied dates.

Practice:

Step 1 prepare ingredients

2. Wash the pig meat and blanch it to remove the fishy smell.

3. Wash carrots, peel and cut into pieces with a hob.

4. soak the dried vegetables for half an hour in advance, wash the lily, apricot, fig and candied dates, and put the meat with the pig in a stew pot and pour in water, which will cover the ingredients.

5. stew for 1 hour, add medlar, season with salt, and enjoy.

This soup is four seasons soup, suitable for the whole family, old and young. Clearing heat and promoting fluid production, clearing heat and moistening dryness to adapt to the crowd.

Tuesday delicious coconut black chicken soup

Ingredients: half black-bone chicken, sea coconut emperor, Polygonatum odoratum, cordyceps, fig, codonopsis pilosula, yam, candied dates, 5 slices of ginger, and appropriate amount of salt.

Practice:

Wash black-bone chicken, cut into pieces, blanch and drain for later use.

Wash and soak Coconut Emperor, Polygonatum odoratum, Cordyceps, Ficus carica, Radix Codonopsis and Rhizoma Dioscoreae for 10 minutes.

Put all the ingredients into the soup pot, add appropriate amount of water, boil over high fire, turn to low heat for 1.5 hours, and add salt before taking out.

Coconut is the fruit of coconut tree, its juice is clear as water, sweet as honey, crystal clear, cool and thirst-quenching; Black-bone chicken contains many amino acids; Coconut black-bone chicken soup can nourish yin and replenish blood, and can effectively resist aging, enhance immunity, invigorate qi and promote fluid production, clear away heat and tonify deficiency, and is suitable for all ages, fatigue, tiredness and thirst.

Lady Enforcers Yuzhu keel soup on Wednesday

Ingredients: keel, Lady Enforcers, sea coconut, Polygonatum odoratum, jujube and golden jujube.

Practice:

1. Wash the materials one by one, stir up the candied dates and soak them in Lady Enforcers and other products for half an hour.

2. blanch the keel with boiling water and ginger slices, and put the soaked materials in a pot with water and blanched keel, with 5-6 bowls of water. After boiling with high fire, turn to low heat and slowly simmer for 2-2.5 hours.

Lady Enforcers moistens the lungs, clears away summer heat and relieves fever, removes phlegm and relieves cough, nourishes and nourishes beauty, Polygonatum odoratum nourishes yin and moistens dryness, and sea coconut moistens the lungs and relieves cough.

Thursday wuzhimaotao chicken soup

Ingredients: five-finger peach, yam, wolfberry, Polygonatum odoratum, candied dates, ginger slices and salt.

Practice:

1. Prepare the required ingredients.

2. Soak the five-finger peach, Polygonatum odoratum, Dioscorea opposita and red dates for ten minutes, and then wash the impurities on the surface.

3. Chop the chicken, blanch it and rinse it.

4. Put all the ingredients in the pot, add ginger slices, add 7 bowls of water and start to cook with medium heat. After boiling, continue to cook for about 10 minutes, turn to low heat and continue to cook for one and a half hours.

5. Finally, add a little salt. (If there is too much oil, skim off the surface oil first.)

Chicken soup with five fingers and peaches is a famous Guangdong soup with good color and flavor. It is moist and delicious, and can strengthen the spleen and stomach, eliminate dampness and sleep, and relax the tendons and collaterals.

Guangdong qingbuliang on Friday

Ingredients: old hen 400g, Dioscorea opposita, Euryale ferox, Lycium barbarum, Chinese caterpillar fungus, Codonopsis pilosula, lotus seeds and figs.

1. First of all, clean the old hen and cut it into big pieces. Blanch and wash.

2. Then wash the yam, Gordon Euryale seed, wolfberry fruit, red dates, lotus seeds, lilies, codonopsis pilosula, coix seed and figs.

3. Then put water in the pot, add ginger, add chicken pieces, boil over high fire, remove floating foam, and turn to low heat for 1.5-2 hours. Finally, just season with salt.

Guangdong’s well-known Qingbuliang chicken soup, Qingbuliang chicken soup is a home-cooked old fire soup in many Guangdong neighborhoods. The traditional Baita mild material is suitable for all ages. "Qingbuliang" has the effects of clearing away heat and toxic materials, invigorating spleen and eliminating dampness. Drinking some Qingbu Liangtang can ensure refreshing and peace of mind.

Saturday flower gum corn sparerib soup

Ingredients: ribs 350g, about 6 pieces of cod glue, one corn, about 15-20 Yao Zhu, 4 red dates, 5 pieces of ginger, and appropriate amount of salt.

Practice:

1. Soak the gelatin in cold water for about 10 hours, put it in a pot, put a few pieces of ginger on it, and steam it for about 20 minutes.

2. Wash and chop the ribs, blanch and drain them for later use.

3. Wash the corn, ginger, yaozhu and all the materials, put them into the soup pot together with the gum and ribs, and add a proper amount of water.

4. Boil over high heat and turn to low heat for 1.5 hours. Add a little salt and serve.

This soup is an upgraded version of the daily corn sparerib soup. It uses 300 scallops and cod glue, which instantly enhances the flavor of the whole soup and is nutritious. Flower glue can enhance the digestion and absorption function of the stomach, stimulate appetite, promote blood circulation, replenish blood, stop bleeding and improve the immunity of the body. It is more suitable as a tonic for people with weak constitution, deficiency of true yin and mental fatigue.

Sunday Morchella Sishen Decoction

Ingredients: pork bone 300g, Morchella esculenta, Pseudostellaria heterophylla, Dioscorea opposita, Euryale ferox, Poria cocos, lotus seeds, candied dates, ginger and salt.

Practice:

1. soak the morel in warm water for a while and let it soak for half an hour.

2. Cut pig bones and wash them.

3. Wash and soak Dioscorea opposita, Euryale ferox, Poria cocos, lotus seeds and candied dates for 10 minutes.

4. Put all the materials into the soup pot and add appropriate amount of water.

5. Boil over high heat and turn to low heat for 1.5 hours. Add a little salt and serve.

The Morchella Sishen Soup contains a variety of hematopoiesis components, which can effectively improve and prevent various anemia. Its nutritional components are not only rich, balanced and reasonable, but also delicious. Benefiting the stomach, helping digestion, resisting virus and improving immunity are suitable for all ages.

The above one-week soup menu meets the needs of a family. It is a customized version of Guangdong soup for fans. Now, as long as it is 128 pieces, it is scientifically matched and balanced in nutrition, and it is suitable for a soup of all ages. For 3-5 people, it is 18.2 pieces per week and 3.64 pieces per day. A few pieces can bring delicious food to every member of the family, bring health and properly cure the heart and stomach of the whole family!

Source/Guangdong soup

Original title: "A big wave of 90 has begun to maintain health! How to keep healthy by drinking soup? 》

Read the original text

　　[Pacific Auto Network New Car Channel] There are not many news that can grab the headlines of Guangzhou Auto Show in the past two weeks. Apart from supermodel wrestling, there is only one electric car that can break 100 in 2 seconds and instantly lose all super-running: a new generation of Tesla Roadster.

　　In the past, many car fans were dismissive of electric cars that claimed to subvert the traditional automobile industry, because in the eyes of our internal combustion engine party, nothing is a car without an internal combustion engine, only an internal combustion engine is fun and has the strongest performance. But now, the acceleration time of the new generation Tesla Roadster in two seconds has made the internal combustion engine party have to re-examine the electric car.

　　Tesla Roadster broke 100 achievements and over 10,000 torques in 2 seconds, which we will explain later. First, let’s take a look at how to buy this outrageous electric car.

Direct link to book a car: Tesla China official website

　　The new generation was officially released at the Tesla Design Center in Hawthorne, California last week. The new car will be delivered in 2020 and has been pre-sold overseas. Judging from the booking amount of 1.33-1.659 million in China District, Tesla is ready to let China buyers "Book the car in full+wait for three years”。 Of course, the final price of Roadster in China will be higher than that in the United States. After all, taxes and fees have not been calculated yet.

　　P.S.　

　　1, Base (basic version)The booking fees at home and abroad are different.

　　2. Please note that even if the full amount is paid, it is only "book",not"schedule”； (The deposit does not have the nature of debt guarantee. If the party receiving the deposit breaches the contract, it only needs to return the deposit it has received without double payment. )

　　3. The Founders Series is limited to 1,000 vehicles. The "sincerity gold" in the United States is the quotation itself. It can be seen that this series is more like a crowdfunding game … …

　　4. Musk announced on Twitter yesterday that completing 0-96km/h in 1.9 seconds is onlyBaseThe performance index of,Founders Series will be more lethal.

　　1.33 million three-year zero interest and zero income to Tesla is to become the first person in this area who can be forced by Tesla sports cars after mass production. We can’t guess the idea of local tyrants. So even if you pay 1.33 million to book a car, can you guarantee that Roadster will be mentioned in three years?

　　With today’s Model 3 as a lesson, I am afraid that local tyrants may not be able to pick up their cars with peace of mind after three years. Tesla’s third-quarter financial report shows that Tesla has only delivered 222 Model 3s, which is far from Musk’s previous claim of delivering 500 models per week.

　　The low production capacity is because the automatic production line of Panasonic, the supplier of power batteries, is not ready yet, which leads to low battery output, which further limits the output of Model 3. Tesla had hoped to produce about 1,500 Model 3 vehicles in the third quarter of this year, but only 260 vehicles were produced. It can be seen that Tesla has already encountered a serious productivity hell.

　　Tesla Model 3′ s capacity crisis has not yet been resolved. Compared with working day and night in the California factory, Musk slipped out of a supercar with exploded parameters.

　　Because Tesla needs money too much, it is necessary to release a new car at this juncture, even if it is not for direct cash flow, raising the price-earnings ratio and boosting the confidence of the capital market. Just looking at the choice of time nodes, Musk is a master of capital.

　　Whether it is Model 3 or today’s new Roadster, Tesla has made products into futures, and released the final product in the form of a prototype three years later. You know, not to mention other high-return investments, even if you put 1.33 million in the bank for regular three years, you can earn at least 200,000 to 300,000, which is equivalent to raising the price in disguise. Whether it is a loss or a gain, only the customer who made the deposit knows.

　　Tesla’s crazy burning of money is worrying Wall Street. According to relevant news, it has obtained more than $3 billion in funds through various channels such as stocks, convertible bonds and bond issuance this year, and it has burned $2.6 billion in the past two quarters alone. It is precisely because of the shortage of funds that Musk hopes to bring new funds to it by issuing bonds.

　　In the fourth quarter, Tesla is expected to invest $1 billion; As of the end of last quarter, Tesla still had 3.5 billion yuan (total cash+cash equivalents). Analysts predict that by the end of the first quarter of next year, Tesla’s book may be only about $1 billion. In order to maintain normal operation, Tesla needs to raise another $2.5 billion to $3 billion.

　　As soon as the launch of the new car at the Tesla Design Center in Hawthorne, California, the mainstream voice of the media was pursuing trucks and the new Roadster. Today, Tesla’s share price has risen from the lowest of $299 when Q3′ s financial report was released to $308.74 (closing price on November 20th), but it has dropped by nearly 21% compared with the all-time high of $389.61 on September 18th.

　　Although Tesla’s electric semi-trailer trucks and the second-generation Roadster have both appeared, investors are still worried about whether Model 3 can successfully complete the mass production target.

　　At present, the market has a broad prospect, but the competitors will not lag behind Tesla in terms of technical ability and capital. Once Tesla’s cash flow continues to deteriorate, it is doubtful whether it can guarantee the current development status.

　　It’s good news that Tesla set up its own factory in China. Musk also made it clear in a conference call that he would not make capital expenditure on China before 2019. Although Tesla is very likely to set up a factory in China independently, it can’t hydrolyze the thirst. At present, the capacity problem of Model 3 is still severe, and the cash flow is further deteriorating.

　　Now it is said that the market share of that windows8 system has risen the fastest, and that it is the win8 system. More and more people install win8 system after being recognized by the market. However, because the time for free upgrade of win8 has passed, many people have not activated the system after installing it. Many functions of the system without activation cannot be used. In order to solve this problem, Xiaobian collected activation codes of various versions of win8 system for everyone to use. With activation code tutorial.

　　I. How to use the activation code, taking win8 Professional Edition as an example:

　　1. Press Win +X at the same time, and then select the command prompt (Administrator).

　　2. Enter in turn at the command prompt:

　　Slmgr.vbs /upk (at this time, the pop-up window shows "Product key successfully uninstalled")

　　Slmgr/ipkw269n-wfgwx-yvc9b-4j6c9-T83gx (pop-up window prompts: "Product key successfully installed")

　　Slmgr /skms zh.us.to (pop-up window prompts: "The computer name of key management service is successfully set to zh.us.to")

　　Slmgr /ato (pop-up window prompts: "Product successfully activated")

　　Second, the activation keys of each version of win8

　　Professional Edition: W269N-WFGWX-YVC9B-4J6C9-T83GX

　　Education Edition: NW6C2-QMPWW-D7KKK-3KGT6-VCFB2

　　Professional edition n: mh37w-n47xk-v7xm9-c7227-gcqg9

　　Enterprise edition n: dph2v-ttnvb-4x9q3-tjr4h-khjw4

　　Education edition n: 2wh4n-8qgbv-h22jp-ct43q-mdwwj

　　Enterprise edition: NPPR9-FWDCX-D2C8J-H872K-2YT43

　　Home Edition: TX9XD-98N7V-6WMQ6-BX7FG-H8Q99

　　Enterprise version lstb: wnmtr-4c88c-jk8yv-hq7t2-76df9

　　Enterprise version lstb n: 2f77b-tnfgy-69f-b8ykp-d69tj

　　The above is how to use the activation keys and keys of each version of win8. Friends who need to activate can refer to this tutorial for operation.

?　　Foreword　　

In 1993, Belgian scientists discovered a new antibody-nano-antibody (VHHs) in camel serum, which is different from the traditional antibody structure and only consists of heavy chain variable regions. Compared with traditional antibodies, nano-antibodies have the advantages of stable physical and chemical properties, low immunogenicity, strong tissue permeability, and can recognize epitopes that traditional antibodies cannot recognize, which makes them a strong and beneficial supplement to traditional antibodies.

Nano-antibody is only composed of heavy chain variable region, with small molecular weight and simple structure, which enables it to realize stable expression in various expression systems. So far, VHHs has been expressed in many expression systems, from prokaryotic cells, yeast, fungi, insect cells, mammalian cell lines to plants. This paper mainly introduces the expression of nano-antibody in different expression systems, and the present situation and development trend in the field of this system.

Prokaryotic expression system　Prokaryotic expression system is the earliest mature protein expression system studied. Obtaining foreign proteins through prokaryotes can obtain gene expression products in a short time, and the cost required is relatively low. The expression system mainly includes Escherichia coli expression, Bacillus subtilis expression and Streptomyces expression. Among them, Escherichia coli system is the most widely used, and the prokaryotic system generally refers to the expression of Escherichia coli.

Escherichia coli expression system　Escherichia coli expression system is the most important expression platform of recombinant protein in laboratory environment, and the expression is controlled by lactose operon. The host of Escherichia coli system has the advantages of fast growth, simple culture, low price, clear genetic background and high protein expression. However, it can not modify the expression product after translation, and the expression product contains a lot of endotoxin and is easy to form inclusion bodies.

Common host bacteria of Escherichia coli expression system

Periplasmic space, as the only compartment with oxidation environment in Escherichia coli, is the most commonly used expression system of nano-antibodies containing specific disulfide bonds. In addition to aerobic environment, there are many partners and isomerases in the periplasmic space, which can promote the correct formation of disulfide bonds and help protein fold. Nano-antibody protein is first produced in the cytoplasmic space, and then secreted into the periplasmic space through Sec pathway, SPR pathway or Tat pathway. After signal peptide is removed by signal peptidase, the target protein can be obtained [1].

Up to now, many VHHs have been successfully expressed in periplasm, some of which can reach tens of milligrams per liter, but there are still a lot of VHHs with low yield. This is because many fusion proteins can not be effectively secreted in the periplasmic space, and cytoplasmic expression seems to be the only expression mode of such fusion proteins [8].

The cytoplasm is a reduced compartment, so that the proteins expressed through the cytoplasm can not be folded correctly, and most of them are inclusion body proteins. The proteins are protected from the degradation of intracellular enzymes and are easy to be separated in high purity and concentration. However, there are many kinds of proteins in cytoplasm, and it is difficult to purify them. At the same time, nonfunctional inclusion proteins need to be completely denatured and refolded to be recovered from these inclusions, but the recovery of VHHs is usually inefficient [1]. In order to solve the problem of cytoplasmic expression, Xue X and others designed a new dialysis-dilution device in 2016. Using this device, researchers refolded VHHs and achieved a recovery rate of 85%, while the conventional dialysis and dilution methods only reached 13%[9]. Bacterial cytoplasm can be an effective compartment to produce correctly folded nanoparticles when expressed by recombinant vectors with sulfhydryl oxidase and disulfide isomerase activities. Larger volume and wider cytoplasm of chaperone/folding enzyme can achieve high yield of nano-antibody [6]. At the same time, double mutants with defects in thioredoxin and glutathione pathways have been developed. Some of these strains have been sold commercially in recent years, such as Origami (Novagen), Rosetta-gami (Novagen) and SHuffle?T7 (New England Biolabs) [10]. Among them, Shufflet 7 overexpresses a critical periplasmic isomerase DsbC, which can effectively catalyze the isomerization of disulfide bonds. Some studies have found that,The activity of isomerase DsbC can increase the yield of functional recombinant protein in periplasmic research [11].　　

Kavousipour Soudabeh and other modified vectors added four different signal peptide sequences to guide the secretion and expression of nano-antibodies, and found that TrBC signal peptide had the best effect [5]. Jianli Yu et al. used repetitive amino acid sequence motifs (GAGAGS) found in silk fibroin (SFP) as a new fusion tag (SF-tag) to enhance the expression of nanosomes in E.coli.. The transcription level of labeled protein is 2.3-7.0 times that of unlabeled protein, and the yield is increased by 2.5-7.1 times [27]. Xi Xie and others tried to select antifreeze protein AXX, and obtained its reverse transcription protein XXA by synthesizing XXA gene, which was used to develop new soluble fusion tags. It was found that the solubility ratio of fusion protein expressed by XXA fusion tag reached 86%, which was much higher than that of other tags [12].

Other prokaryotic expression systems　Lactic acid bacteria are the most common intestinal probiotics, which are generally considered to be harmless to human body and are very suitable for the production host of therapeutic molecules. However, the existing expression data show that the yield of VHHS produced by lactic acid bacteria is low, about 1-3mg/L, which is much lower than that of Escherichia coli [13,14]. Lactobacillus rhamnosus DSM14870, as a normal flora in human vagina, can express HIV antibody in the form of soluble or cell wall anchor fixation, and is a suitable expression host for HIV antibody [2]. When Bacillus brevis choshinensis was used for 3L fed-batch fermentation, the yield was as high as 3g/L[15]. This system is easy to manipulate genes, has no endotoxin and shows weak protease activity, so it is a very noteworthy system.

Eukaryotic expression system　

Due to the defects of prokaryotic expression system, such as difficult purification of inclusion body protein and incomplete protein modification, people began to use eukaryotic expression system to study genes. At present, eukaryotic expression systems commonly used in genetic engineering research include yeast expression system, insect cell expression system and mammalian cell expression system.

Yeast expression system　Yeast system has clear genetic background, simple operation, easy genetic operation, perfect eukaryotic protein expression control system and advantages of both prokaryotic and eukaryotic expression systems. Yeast eukaryotic protein expression systems include methanol yeast expression system, Saccharomyces cerevisiae expression system, Schizosaccharomyces expression system and Kluyveromyces expression system [17].

Saccharomyces cerevisiae is the first yeast fermented by human beings, which has no specific virus and low cost. At present, it is the largest yeast expression system in shake flask culture, with the output exceeding 100mg/L[16]. Some amino acids in VHHs can participate in the recruitment of molecular chaperones in endoplasmic reticulum, thus narrowing the difference of molecular chaperones between Saccharomyces cerevisiae and mammalian cells, which is beneficial to the folding kinetics of nano-antibodies and improving the yield of nano-antibodies in Saccharomyces cerevisiae [17]. The protein expressed by Saccharomyces cerevisiae is prone to hyperglycosylation, and a large number of endogenous proteins are produced during fermentation, so the secretion efficiency of the target protein is low [1]. Considering the above problems, Saccharomyces cerevisiae is gradually replaced by methanol yeast represented by Pichia pastoris.

Pichia pastoris expression system is an efficient expression system of foreign proteins. This microorganism does not accumulate toxic ethanol, but only secretes a few endogenous proteins, and its glycosylation degree is the same as that of mammalian cells, so its immunogenicity is weak, so it is the most commonly used eukaryotic expression system. Pichia pastoris has a strictly regulated methanol inducible AOX1 promoter, which is suitable for high expression of foreign proteins [1]. But methanol is a highly toxic and dangerous chemical product. The GAP promoter without methanol induction is used instead of AXOI, and the carbon source does not need to be changed during the culture process, so the operation is simpler and the time for foreign proteins to reach the peak level can be shortened. The yield and affinity of nanoparticles produced by this system are similar to those produced by methanol-induced expression. It has been studied that the yield of nano-antibody expressed by this system in shake flask is 51.71mg/L[18] and 16mg/L[19].

Fungal expression system　

Some filamentous fungi, such as Trichoderma and Aspergillus, can secrete a large number of foreign proteins in the culture medium. However, the fungal system not only expresses protein of interest, but also produces many proteases, which affects the yield. Joosten et al. obtained only 1.5 mg/L VHHs in Aspergillus awamori medium. After adding 0.25% BSA, it reached 7 mg/L. It is reported that more than 80% of VHH is degraded or adhered to the cell wall compared with endogenous xylanase secreted efficiently in this system, and researchers believe that the low secretion efficiency of VHHs is the bottleneck of fungal expression [20]. In order to overcome this limitation and protect VHHs from degradation, other researchers fused VHH with the high amylase A signal sequence (sTAA) of Rhizopus oryzae lipase (N28) and the N-terminal 28 amino acid fragment (N28), so that the highest yield of VHHs expressed in Aspergillus oryzae reached 73mg/L, which was 300-800 mg/L lower than that of Escherichia coli [21]. The final yield of GlaB-VHH fusion protein in Aspergillus oryzae can reach 610mg/L, which provides the possibility for high expression of nano-antibody, but its stability needs to be verified [22]. In addition, VHH[23] was expressed by maize head smut in unconventional secretion pathway.

Insect cell expression system　

Insect cells are a widely used eukaryotic expression system, and recombinant baculovirus vectors (such as AcMNPV) can specifically transfect insect cells. Its survival in nature is short, and the strong promoter is inactivated in mammals and cannot infect vertebrates, so the recombinant baculovirus is safe for human beings. Proteins with complex structures under the control of strong promoters may not be able to form correct folding and post-translation modification, which may lead to the aggregation of target proteins and reduce antibody production, so the expression yield of VHHs in this system is much higher than that of other types of antibodies [17].

Gómez-Sebastián and others used the improved baculovirus expression system (IBES? technology) to express VHHs for the first time. The chigger larvae were inoculated with different recombinant baculovirus inoculation methods. The yield of VHH extracted from larvae is 257 mg/L, and the recovery rate after purification is 50%[7]. When expressing anti-endothelial cell selection protein, Drosophila cell SC-2 secreted 12-14mg/L, but the target protein was not detected when expressed in Escherichia coli at the same time, which may be related to the processing and modification ability of insect cells [26].

Mammalian cell expression system　Mammalian cells are the best expression system for therapeutic antibodies, because the produced antibodies have the weakest immunogenicity. At present, the main cell lines include CHO, NS0, Sp2/0, HEK293 and per C6 [17]. Among them, CHO cells grow rapidly and adapt to serum-free suspension culture, which is the most popular host. They can express VHHS up to 100mg/L in shake flask [24]. HEK293 cells are more commonly used in the laboratory because of transient transfection and semi-stable expression, because they require less time and labor. However, monoclonal antibodies approved for the treatment of human diseases are only produced by CHO, NS0 and SP2/0. So far, HEK293 cells have not been used to produce VHHs, and there are few reports about producing antibody fragments by this method [17]. In most literatures, CHO is used to produce antibody fragments. Up to now, 50% of the therapeutic proteins produced in industry are still produced by CHO [1]. The production costs associated with mammalian cell lines are much higher than those associated with any other laboratory research platform.

Common host cells of mammalian expression system

Plant expression system　Compared with other expression systems, plant production platform (stable and transient expression system) is cheap, safe, capable of folding and assembling complex proteins and post-translation modification, easy to expand, and unaffected by human pathogens and diseases. It is an ideal protein expression host and is usually used as an alternative method for mammalian expression systems. In addition, plant expression products can be concentrated in specific organs, such as leaves or seeds, and can be taken orally directly. Tobacco leaves are the most common hosts for expressing VHHs, while the proteins expressed in Arabidopsis and rice seeds have attracted much attention because of their direct oral input of VHHs[1]. The stable transformation methods commonly used in plant system expression have some problems, such as the recombinant gene escaping to nature or the time-consuming transformation. Therefore, some studies have proposed the transient expression system of plant virus expression vector as an alternative method, and the nano-body content with correct conformation reaches 0.45% of the total soluble protein [25].

Present situation and development of nano-antibody expression　

Escherichia coli expression system is the most commonly used expression system of nano-antibody, which has two expression modes: periplasmic expression and cytoplasmic expression, each with its own advantages and disadvantages. Some oxidation engineering strains have been modified, but more experimental support is needed. In addition, various methods of modifying vectors, such as adding labels or signal peptides, have been put forward and have been verified by experiments. Compared with Escherichia coli, Pichia pastoris has excessive glycosylation, which may lead to immunogenicity and ultimately affect its clinical application. The expression systems of fungi, insects, plants and mammals are much more complicated than prokaryotic and eukaryotic expression systems, and are generally not easily adopted after considering the production time, cost and laboratory conditions.

Escherichia coli system is fast, convenient and cheap, and it is the first choice for VHH expression under most laboratory conditions. When some types of VHHs can’t be successfully produced in E.coli, other systems will be used instead.

List of nano-antibody expression

　　【 Different opinions 】

　　Recently, in order to comply with the audience’s opinions and meet the audience’s expectations, the "changing while broadcasting" that timely modifies the relevant content according to the audience’s feedback and public opinion calls during the broadcast process has become a new operation mode of drama series in video website communication. From "Do you know whether it should be green, fat, red and thin" to "slow-motion play" in the new edition of "Eternal Dragon Slayer", there are various forms of expression. This rapid response mechanism is being used by more and more drama producers after achieving some visible communication effects, and has won many praises from the industry and the audience.

　　For creators and communicators, the behavior of "changing while broadcasting" represents the communication concept of respecting the audience, and also shows the creative attitude of being pragmatic and striving for perfection. Compared with those practitioners who regard film and television creation as a "one-shot deal", who know that there is a mistake but don’t admit it, or even face criticism and questioning, the behavior of knowing the mistake and correcting it in time is worth encouraging. However, this kind of "after-sales service" is, after all, a new phenomenon of production and broadcasting emerging in the process of rapid development of the industry. Most viewers don’t know much about it, and its operating mechanism is not perfect, so there are still some possible negative factors hidden in the actual operation process, which need to be vigilant. On the one hand, this model will subtly encourage the creator’s luck, that is, "it doesn’t matter if it is casual and rough in the original creation, even if there is an error, there is still room for revision in the broadcast process." Once such a psychological formula is formed, it will undoubtedly do harm to the development of the industry. On the other hand, respecting the audience does not mean blindly satisfying and catering. The audience can design whatever bridge they want to see and arrange whatever ending they want. In fact, it is an irresponsible cramming creation. Regardless of the ideological theme or aesthetic taste, the audience needs more guidance, and there is no need to say anything about it. In most cases, the forced temporary modification behavior in the process of broadcasting is a last resort.And is not a conventional operation mode. Creators should feel guilty about the lack of rigor and thoughtfulness when they started, and learn from it, so as to make it happen again. This must not be used as an excuse and retreat for inferior creation. And those so-called "at the request of the audience" to modify the fate of the characters and the ending of the story are even more cautious. After all, literary and artistic creation that appeals to people’s hearts is serious. If it caters to or even condescends to the tastes of some audiences at the expense of distorting the original intention of creation, in essence, it will make commercial benefits overshadow social benefits and abandon the basics.

　　In addition, there is a more dangerous business appeal to be vigilant. The producers of individual dramas know that their works have serious injuries and "slots", so they not only broadcast them directly without modification, but also hype these issues as gimmicks to generate public opinion topics to achieve the purpose of attracting attention. Then, we made a big fuss about the "after-sales service" while broadcasting, to show the film’s "attitude of admitting mistakes" and "speed of correcting mistakes", so that the audience could feel the satisfaction of being valued, and they seemed to enjoy a high-level drama-watching experience with high participation and strong interaction. This behavior takes the mode of "changing while broadcasting" as a marketing means and commercial trick to capture the audience, which makes the creation and dissemination of works taste worse and worse.

　　As far as the operation mode of "modifying while broadcasting" is concerned, domestic dramas are still in the primary stage of "correcting mistakes and filling gaps", while some countries with mature TV drama industry such as Europe, America, Japan and South Korea have already realized the operation paradigm of "writing, filming, broadcasting and modifying while broadcasting". In recent years, an interactive creative mode known for immersion and interactivity has become popular in the industry. This kind of work can be regarded as a mixture of watching and participating. It tries to give full play to the dynamic role of the audience, so that they can make choices at some key nodes in the process of watching, thus having strong control over the role decision in the work, and finally determining the plot development and the fate of the characters in the drama. Although all these measures are more rigorous and mature compared with the "broadcast while changing" mode, they fully integrate the wisdom of the audience into the drama creation, so that the works can basically keep pace with the market demand, but they have not fundamentally solved the disadvantages and hidden dangers of the "broadcast while changing" mode. If the operation paradigm of "writing, filming, broadcasting and changing" and interactive works become the possible development trend of China drama production and broadcasting in the future, then relevant practitioners need to keep a calm, rational and prudent attitude towards them and guard against their dislocation and deviation in practice.

　　Creators and communicators should at least be soberly aware of the following points: First, the original intention of "modifying while broadcasting" should not only be positioned at the omissions and deficiencies in the process of modifying and creating, but should achieve the balance between audience satisfaction and market ratings through deeper communication and reception interaction; Secondly, we should prevent the phenomenon of catering to the audience’s tastes to the neglect of the establishment of positive values and the original aesthetic style and artistic characteristics of the works, and grasp the degree of satisfaction and guidance under the principle of "social benefit first"; Third, the ultimate goal of "broadcasting while changing" is not to attract eyeballs to earn money, but to create a benign environment for full communication with the audience, to maximize the audience’s wisdom to bless the quality improvement of the drama, and to make the works more artistic vitality and market communication; Fourthly, we should devote ourselves to constructing a long-term mechanism of self-improvement and self-transcendence of drama series, so that the works can continuously gain the internal drive to the road of quality products in different links of creation and communication, and help the whole industry to mature and prosper.

　　(Author: Yan Wei, director and editor of China Television Arts Committee)

　　China Overseas Chinese Network July 27th According to the report of Spain’s Oulang. com, the Spanish Chinese 100-yuan store food stores are facing competition from a large number of small supermarkets, but at the same time, many Chinese have changed their careers and directly joined the chain operation to become the owners of small supermarkets. The large number of small supermarkets has attracted the attention of statistical agencies.

　　According to "american retail", there were 550 small supermarkets opened in Spain last year, most of which were small fast-selling supermarkets under big chain brands, such as Carrefour Express. This is closely related to the change of residents’ consumption habits. More and more people advocate fast, which provides a market for small supermarkets in residential areas.

　　"Spaniards are gradually giving up going to traditional markets and large supermarkets in the suburbs, but choosing online shopping or small supermarkets in residential areas." In the past ten years, this supermarket revolution has been replaced unconsciously. However, the origin of all the changes starts from 2009. From that year, the commercial law changed, it became easier to open a small convenience supermarket, and the government also encouraged the development of small commodity trade. In 2013, the People’s Party government at that time passed the Business Freedom Emergency Act, which once again contributed to the opening of such commercial supermarkets. Subsequently, chain operation rose rapidly, and in 2017, it began to enter a period of explosive growth.

　　Faced with this phenomenon, some analysts believe that small supermarkets are struggling to survive in the gap between large supermarkets and hundred-dollar stores, while others believe that this has brought crisis to Chinese hundred-dollar stores. At present, consumers are more and more interested in buying goods near their place of residence, which requires higher and higher speed of purchase. In addition, the requirements for quality have not decreased. Therefore, this is an excellent opportunity for small supermarkets. On the other hand, many small express supermarkets have become the pick-up points for online shopping and play an important role in the whole online shopping chain. (Xiaoxin)

Don’t worry, generally speaking, Korean cars will get a discount of 30,000 to 50,000 soon. Don’t worry, generally speaking, Korean cars will get a discount of 30,000 to 50,000 soon. Generally speaking, Korean cars will get a discount of 30,000 to 50,000 soon. Don’t worry, generally speaking, Korean cars will get a discount of 30,000 to 50,000 soon.

Recently, Geely’s Galaxy brand has once again exerted its strength, and the new model Starship 7 EM-i has been heavily listed. Galaxy Starship 7EM-i was built based on the new GEA architecture, and it was the first to build a new generation of Raytheon EM super hybrid technology. With the iteration of the architecture and the improvement of product strength, it was immediately popular in the market. From the dealer’s point of view, the popularity of Starship 7 EM-i is comparable to that of E5 in the same period of listing, and both products and pricing exceed expectations. Geely Galaxy brand is expected to continue to create new explosions.

Since the beginning of this year, Geely automobile sales have performed well. In terms of exports, Geely has successfully grasped the export dividend by virtue of its factory layout in Southeast Asia, Europe, North America and other places. The cumulative export volume from January to September this year increased by 61.9% year-on-year. In the domestic market, the sales of Yinhe E5, Krypton 7X and Geely Xingyuan have further consolidated Geely’s position in the domestic market. As the first profitable model of Galaxy brand, the success of Yinhe E5 not only proves that Geely has obviously improved in market demand control and platform cost reduction, but also laid a solid foundation for the launch of subsequent models.

In terms of technical architecture, the launch of the new GEA architecture enables Geely to meet the needs of pure electricity, hybrid and other power forms, and at the same time, it has a high degree of modularity and reduced development costs, further improving the cost performance of products. The upgrade of Raytheon hybrid system has enabled Geely to lead the industry in key parameters such as thermal efficiency, battery life, power loss and fuel consumption per 100 kilometers, bringing consumers a better driving experience. Thanks to the overall upgrade of the Galaxy brand in terms of architecture, power system and models, the fundamentals of Geely Automobile are expected to continue to exceed expectations in 2025.

With the launch of many new hybrid cars such as Starship 7 EM-i and modified L6&L7 under the Galaxy brand, the market share of Geely Automobile is expected to further increase. Especially in the price range of 100,000-200,000 yuan, Geely’s hybrid products have a longer wheelbase and more space, and their pricing is lower than that of joint-venture oil vehicles, which will effectively seize market share.

According to Zhongtai Securities’s in-depth research, Geely Automobile is welcoming a new round of strong product cycle. With the continuous deepening of internal resource integration, its performance is expected to continue to grow, and it will continue to be given a "buy" rating. # Galaxy Starship 7 EM-i’s explosive potential highlights # # Galaxy Starship 7 EM-i’s delivery exceeded 6,500 units in 7 days #

Interaction of social media: the close connection between stars and fans

In this digital age, social media has become an important platform for interaction between stars and fans. Bao Beier and Cai Ming are active in daily social media, sharing their life and work trends with fans from time to time. This kind of interaction not only makes fans feel close, but also makes them full of expectation and curiosity about the upcoming film.

After the release of the file, there were a lot of comments on social platforms in Bao Beier and Cai Ming, and many fans expressed their support and expectation for the film. Among them, all kinds of creative discussions and fans’ positive suggestions have emerged, forming a good communication atmosphere. This kind of interaction has undoubtedly enhanced the popularity of the film and become an important boost for film promotion.

Comprehensive Evaluation: Looking at "Can’t Stop" from Various Perspectives

Although the withdrawal of the film has caused a lot of controversy, from many angles, it may also be a strategic adjustment made by the film team to present better works. Industry experts believe that although the market competition is cruel, most viewers are more willing to wait for an excellent work that has been carefully polished. From the production of Bao Beier and Cai Ming, their efforts and expectations have been rewarded accordingly, and the industry generally has high hopes for the performance of the two protagonists in the film.

In short, although "Can’t Stop at All" has not yet met the audience, there are various indications that this intergenerational partnership between Bao Beier and Cai Ming will arouse more ripples in the future film and television circle. Whether from the emotional depth of the film or in the face of increasingly complex social relations, this film has the potential to become an important work reflecting family and humanity at present. With the announcement of the new schedule, I believe more audiences will look forward to this film full of laughter and thinking, and look forward to the new experience and touch brought by Bao Beier and Cai Ming.

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