Today, the problem of bacterial drug resistance has launched a major challenge to the global public health field.

　　Every year, 700,000 people worldwide die from drug-resistant infections, and 230,000 newborns die. Studies have shown that bacterial drug resistance mainly comes from the abuse of antibiotics, including both human treatment abuse and excessive use of animals.

　　Antibiotics are "double-edged swords"

　　Antibiotics are a kind of secondary metabolites produced by microorganisms (including bacteria, fungi and actinomycetes) or higher animals and plants in the course of life, which have anti-pathogen or other activities and can interfere with the development function of other biological cells. At present, the commonly used antibiotics in clinic are genetically engineered bacteria, extracts from culture medium and compounds synthesized or semi-synthesized by chemical methods. If used reasonably, antibiotics are the bane of bacteria. If abused, bacteria will "immunize" it and become super bacteria, resulting in the final incurable infected person.

　　Superbacteria are bacteria that can’t be killed by using all antibiotics. This kind of bacteria can show resistance to almost all kinds of antibacterial drugs, even to powerful antibiotics such as carbapenem antibiotics and vancomycin, and some of them also show multiple resistance. Because patients are often unable to produce anti-bacterial antibodies from their own bodies after infection, the mortality rate is greatly increased.

　　Powerful antibiotics are usually regarded as the last line of defense against superbugs. Why is it so vulnerable? This will start with our daily medication habits. Generally speaking, we don’t have to use antibiotics in the face of common germs, but patients often ask doctors to cure them as soon as possible, so doctors will habitually use antibiotics in the face of minor illnesses. However, after taking the medicine, patients usually don’t use up all the drugs in a series of courses, and they will stop taking the medicine when their condition improves slightly, which is the main reason for the drug resistance of bacteria. In addition, excessive use of antibiotics in poultry and livestock feed is also a major cause of the outbreak of superbugs. At present, 80% of antibiotics in the world will flow into animal feed, and superbugs will be excreted with animal excrement, and these bacteria have evolved enough drug resistance in animal gallbladder before being excreted.

　　For example, a salmonella that can hardly be subdued by antibiotics can easily spread between people and animals. At present, we can only passively defend against this superbug, but we can’t actively attack it. The main source of this superbug is almost all the intestines, and we are all likely to come into contact with these bacteria that were originally parasitic in the intestines. The World Health Organization admits that it is a very difficult clinical problem that Enterobacteriaceae is resistant to many commonly used and widely effective antibiotics.

　　The influence affects all walks of life. In fact, bacterial drug resistance is not a problem that only exists in the health field. Statistics show that between 2000 and 2014, the standard dose of antibiotics used worldwide has increased by 50%. A research report published by the World Bank on September 19th, 2016 shows that by 2050, the medical expenses of drug-resistant infections will account for 1.1% ~ 3.8% of the world GDP. According to the current development trend, by 2050, the problem of bacterial drug resistance will kill 10 million people every year, and superbugs will also lead to global economic losses of $6.1 trillion. At the same time, problems such as labor shortage, rising medical costs and shrinking market will follow. At that time, the world economy may face a more serious blow than the 2008 financial crisis, especially for developing countries. By 2050, it is expected that the GDP of these countries will decrease by 5% as a whole.

　　According to the research results of the European Bureau for Disease Control, the development speed of modern medicine is nothing compared with the speed of preventing bacterial drug resistance and bacterial variation. The resistance of human body to antibiotics is increasing, and the tools that human beings can use to deal with drug-resistant bacteria are almost used up. If market forces are allowed to allocate resources by themselves, it is impossible to complete the research and development of a new generation of antibiotics on time, and the candidates for research and development of antibiotics will be basically exhausted.

　　The deadliest superbug.

　　On February 27th, 2017, based on the clinical drug resistance, the mortality caused by drug resistance, the frequency of people’s infections and the burden of these infections on the health care system, the World Health Organization released the list of 12 superbugs with the highest priority for the first time. They are Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacteriaceae, Enterococcus faecalis, Staphylococcus, Helicobacter pylori, Campylobacter, Salmonella, Neisseria gonorrhoeae, and Campylobacter. The researchers classified them into three categories according to their degree of danger: emergency, high priority and medium priority.

　　At present, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacteriaceae have been listed in the emergency level. These highly pathogenic bacteria will not only cause drug-resistant infections, but also bring a heavy burden to the existing medical system and take the lives of patients. Among them, Acinetobacter baumannii is resistant to carbapenem antibiotics, which is easy to cause serious infections in hospitalized patients, such as pneumonia, wound or blood infection. Pseudomonas aeruginosa is resistant to carbapenem antibiotics, which can easily lead to rash and ear infection in healthy people, and also lead to serious blood infection and pneumonia in hospitalized patients. Enterobacteriaceae bacteria inhabit human intestines and are resistant not only to carbapenems but also to cephalosporins. In 2014, among the samples of Escherichia coli (a common intestinal bacteria) collected by the World Health Organization, penicillin was ineffective in 60% of the samples, and about 25% of the samples were resistant to one or two other commonly used antibiotics.

　　Enterococcus faecalis, Staphylococcus, Helicobacter pylori, Campylobacter, Salmonella and Neisseria gonorrhoeae are classified into high priority categories, and their main feature is that they are easy to cause infection in healthy people. At present, there is no particularly effective method to combat this kind of infection. The remaining Streptococcus pneumoniae, Haemophilus influenzae and Shigella are given medium priority. The resistance of these three pathogens to existing antibiotics is still increasing, among which Streptococcus pneumoniae, which is insensitive to penicillin, is easy to cause pneumonia, ear sinus infection, meningitis and blood infection.

　　"Drug-resistant bacterial infections are posing a serious threat to our health care system today," said the head of the drug-resistant infection division of Wellcome Trust, a British medical charity. "The list of superbugs made this time is very important to guide the research and development of antibiotics against drug-resistant bacterial infections. If new antibiotics are not developed, 10 million people will die every year because of drug-resistant bacteria infection. In addition, if we fail to develop new drugs against deadly infections, then some treatments and means that could have saved lives, such as chemotherapy and organ transplantation, may also induce death. "

　　Superbacteria ravage many countries.

　　Recently, the European Bureau for Disease Control issued a warning against the current situation of bacterial drug resistance: a superbug is sweeping across Europe. In a physical examination report on animals and food published by the European Union, the name of bactericidal antibiotic clarithromycin appeared for the first time, which is almost the last line of defense antibiotic for those infected by super bacteria. Inspectors can find traces of them in animals and food, indicating that some people have easily used such drugs for some reasons.

　　The report also pointed out that in the pork tested in Europe this time, the staff have found a very small number of drug-resistant Escherichia coli. If these Escherichia coli or Salmonella are transmitted to humans by pigs or cattle, then we will not have any effective antibiotics to deal with them. The report also shows that people in northern and western European countries are generally less resistant to bacteria than those in southern and eastern Europe, which is probably caused by the abuse of antibiotics.

　　At present, Russia is being invaded by superbugs. Scientists have found three kinds of bacteria on the list of superbugs of the World Health Organization in 15 big cities, including Moscow and St. Petersburg. "Three kinds of superbugs were first discovered in the laboratory of St. Petersburg Institute of Children’s Clinical Infectious Diseases," pointed out Yuri Robzin, an academician of the Russian Academy of Sciences. "They can cause abscess, meningitis and septicemia. If we don’t take action now, in the next 10 to 15 years, human beings may die from the humble acne suppuration. "

　　On May 26, 2016, US health officials reported that the first case of bacterial infection resistant to all known antibiotics had been discovered in the United States. The patient was a 49-year-old woman from Pennsylvania. At that time, she went to the clinic because of urinary tract infection, and the doctor used colistin, an antibiotic specially used to fight "nightmare bacteria", but the condition was not controlled. The journal of American Society for Microbiology, Antimicrobial Agents and Chemotherapy, published a report on this infection case. The report said that this superbug itself was first infected by a small DNA molecule called Plasmid, and the plasmid carried the mcr-1 gene that was resistant to colistin. "This shows that there are truly pan-drug-resistant bacteria," warned Tom Freeden, director of the Centers for Disease Control and Prevention (CDC). "This is the first case of mcr-1 reported in the United States. We face the risk of being in the post-antibiotic world. If this super bacteria is allowed to spread freely, it may’ devour’ everyone in the future. "

　　Real countermeasures

　　However, some medical experts have pointed out that the term "superbugs" has actually existed for many years, and some superbugs have even become very common. But in our environment, superbugs are still a minority after all, so you don’t have to panic too much.

　　Facing the challenge of superbugs, we should change our thinking: instead of investing more powerful antibiotics to "exercise" them, we should let them return to the ecological competition of the original colonies. Among bacterial colonies, if there is no selective pressure of antibiotics, it will not optimize the "particularly powerful role."

　　In addition, we should strictly abide by the guidelines for the use of antibiotics in clinic: try not to use antibiotics for patients with bacteria who have no signs of infection. In fact, without the intervention of antibiotics, drug-resistant bacteria are more likely to be replaced by wild drug-resistant bacteria and then disappear from the human body. Abuse of antibiotics will only "strengthen" the strength of drug-resistant superbugs while killing other bacteria. Therefore, adhering to good hygiene habits and striving to improve physical fitness are the truly effective ways to avoid bacterial infection and combat superbugs. Although the iterative update of antibiotics can control the temporary disease invasion, it also gives birth to more powerful drug-resistant bacteria. I would like to ask, in the long run, when is the head?

　　CCTV News:According to the news released by the Sri Lankan police on April 22, the death toll caused by the explosion attacks in Sri Lanka on April 21 has risen to 290, and another 500 people have been injured. The Sri Lankan security department has arrested 24 suspects involved in the explosion. The Sri Lankan government said that most of these explosions were suicide attacks. After the attack, Sri Lankan President Sirisena issued a statement calling on the people to remain calm and cooperate with the official investigation. Sri Lankan Defense Minister Ruwan Wijewardene said that the attack that day was a "terrorist attack". Colombo reported another suspected explosion on April 22, and the ninth explosion detonated explosives for the police.

　　On the afternoon of April 22nd, the Sri Lankan Cabinet held a press conference on the serial bombing attack in Sri Lanka on the 21st. A spokesman for the Sri Lankan cabinet confirmed at the press conference that the Sri Lankan security department had intercepted relevant information about 10 days before the explosion. The intelligence showed that extremist organizations might launch suicide attacks on many targets in Sri Lanka, including hotels, scenic spots and churches. The Sri Lankan government apologized for failing to take timely measures to avoid this tragedy.

　　Li Wei, an expert on counter-terrorism: In counter-terrorism work, counter-terrorism intelligence is the most important link to prevent terrorist attacks, and it is actually the most difficult aspect to obtain intelligence. Sri Lanka actually had some relevant information ten days before this serial explosion, and we also made some analysis on this information. Personally, I think, as a relatively complete intelligence chain, it lacks unknown factors. This terrorist attack may involve too many areas, and I don’t know which buildings, sensitive buildings or dignitaries or politicians to protect, which puts forward great difficulties. What is it lacking most? Who could carry out such a terrorist attack? Without this, it will be very difficult to prevent, so such a terrorist attack also presents a situation that cannot be prevented.

　　Secondly, this serial bombing attack in Sri Lanka presents one of the biggest characteristics. In the past, it was a decades-long civil war between the LTTE and the Sri Lankan government, including some sporadic violent attacks since then. Its target and the way of committing crimes are very different from this serial bombing. Therefore, it is classified as Sri Lanka. This terrorist bombing is the internationalization of local terrorist attacks. Why is it international? Because of the targets it targets, the targets it attacks, and the way it commits crimes, it can be seen in some hot spots of terrorist activities outside Sri Lanka. A church in Egypt was attacked by terrorists, and so was a church in Surabaya, Indonesia. Attacks on five-star hotels, including Marriott in Pakistan, Marriott in Indonesia, Lisen Hotel in Mali and these high-end hotels in Somalia, are consistent with the extreme ideology advocated by international terrorist forces and the goal of hate conflicts they want to create, which is its most prominent feature.

　　The second feature, just now we heard at the press conference that NTG, an extremist organization in Sri Lanka, was behind this crime. It is certain that NTG is involved, but we should also see that another factor of the internationalization of this terrorist attack should be influenced by extremist organizations in the Middle East. Seeing some suicide bombings, this technique can be seen from outside Sri Lanka. In the process of searching the houses of these people who carried out terrorist attacks and bombings, some propaganda materials and some demagogues associated with extremist organizations in the Middle East were also found. Therefore, from this point of view, the serial terrorist attacks and bombings in Sri Lanka are definitely not only the work of local extremist organizations, but also closely related to extremist organizations abroad, especially in the Middle East. This also reflects that international counter-terrorism, especially the counter-terrorism of a country, is difficult to rely solely on the counter-terrorism of a single country. International counter-terrorism must be United, including opposing local terrorism and international terrorism, so as to curb the threat and harm posed by terrorism to human society from a larger aspect.

Author | Li Beichen

Source | Whale Fall Business Review

As we all know, today’s Chinese music scene is called "Shaking".

At the end of last year, Tencent Music made the top ten hot songs of the year, namely: Cloud and the Sea, Bai Yueguang and Zhu Shazhi, Gossip of the Prodigal Son, Dream that Can’t Wake Up, Treading on the Mountains and Rivers, Qian Qian Never Will Fall, Shepherd of Coco Tuohai, Emptying, and Obsession.

Almost all of them are Tik Tok Divine Comedy.

Yes, the most popular part of Chinese music has become the accompaniment of Tik Tok.

In the face of traffic, QQ Music and Netease Cloud Music also chose to bow their heads, with a special list of popular songs in Tik Tok, and QQ Music regarded "Tik Tok Hot Search" as a recommended option for searching songs.

Whether you like it or not, the subversion and controversy brought by Tik Tok to China’s music industry have become increasingly apparent. Those Tik Tok Divine Comedy haunts your mind, which has made the music market in China undergo an unpredictable evolution.

Obviously, Byte himself realized this, and then suddenly realized: "Why should I give up my own traffic?"

So they left the stage and entered the music market in person, which may completely change the appearance of the music market-and even the whole "music".

Traffic coordination in ByteDance

From the moment of its birth, Tik Tok has a close relationship with music-Tik Tok has a "sound" in his name, and the icon is also a note.

Looking back, Tik Tok’s predecessor, A.me, positioned itself as "short music video", which was quite similar to musical.ly, which was popular among American teenagers at that time, and created short videos by guiding users to lip-synch with music or dance. By the end of 2017, musical.ly had more than 200 million registered users.

Also in 2017, musical.ly was acquired by ByteDance, and then merged into the overseas version of TikTok in TikTok, which brought many active users and creators to TikTok and laid a good foundation for its rapid expansion in the global market.

ByteDance’s "formal" entry into the music market began two years ago.

In March, 2020, Byte officially launched the music playing software Resso in India and Indonesia. You can understand it as "Tik Tok in Music". Once the App is opened, it will be personalized song recommendation. If you don’t like this song, you can swipe the screen up and down like a Tik Tok and quickly cut to the next one.

Resso has been online for half a year, and the download volume has exceeded 15 million times. With this experience, in 2021, a music division was established within Byte, and a key task was to open up the music community of Resso and TikTok.

However, due to various reasons, Resoo has not expanded its territory to more countries and regions. At present, only a few countries such as India, Indonesia and Brazil are open.

In terms of music, perhaps the next force point of Byte is the domestic market.

In the spring of 2022, ByteDance launched an online music app-soda music in China.

The interface of soda music is very similar to Resso, which also uses sliding up and down to cut songs. Compared with the familiar left and right key switching, sliding up and down can greatly improve the speed of cutting songs. Under this interactive logic, people are not so much listening to songs as "brushing songs".

More importantly, according to media reports, most of the data of soda music and Tik Tok are synchronized. Soda music can help people to collect the divine comedy heard in Tik Tok synchronously, and find more similar songs in ByteDance’s proud algorithm recommendation.

Byte’s intention to engage in music is obvious, that is, to cooperate with the flow of Tik Tok and soda music.—— It is foreseeable that the soda concert will be more deeply bound with Tik Tok to form a closed loop of flow: Tik Tok can hatch the Tik Tok Divine Comedy and guide it to soda music; Those minority songs in soda music can also be hatched into "soda divine comedy" with the help of recommendation algorithm, which can be used to feed back short video with vibrato and become Tik Tok divine comedy, thus further consolidating Tik Tok’s position in the field of short video.

It does look wonderful.

In the future, music will eventually become shorter.

As for the penetration of bytes into the music field, what it means to the music market in China is a matter of opinion.

Please note that this article has no intention to talk about the vulgarization tendency of Chinese music. After all, there is no need to argue about the reality. The whole Chinese music presents a dangerous prosperity that will collapse once the coarse gravel foundation is removed. Huang Shujun has a song called "Change 1995", and there is a lyric that won my heart: "Only pop, no music, I think it is a good thing that you are out of sight."

So today, we are out of sight, avoiding "music quality" and talking about "music length".

Have you ever thought about it,When music can be "brushed", people will inevitably leave less and less time for a song, and they will become more and more stingy, so that they will never give you "time for a song" again.

Yes, now is not the era of "slow in the past". In the past, everyone was sincere: a song is a song; Movies, one is another. This is not the case now. People’s interest cycle is getting shorter and shorter, and the content carrier is also getting shorter and shorter, so that they can be embedded in people’s increasingly fragmented nothingness life at any time.

Therefore, just as people have become more and more impatient to watch the whole movie and are used to watching the short video of "watching a movie in X minutes", people have become more and more impatient to listen to the whole song and are used to going straight to the climax and only listening to the chorus (especially for new songs).

I even think,One day in the future, the online music platform will be completely "chorus", that is, every song that people brush will only have a climax.

If this is the case, then this new technology carrier will change the music-the time of a song is bound to become shorter, and the "memory points" such as melody and rhythm are bound to become more accurate-as much as the music was changed when vinyl was born.

You know, nowadays, a song usually lasts only a few minutes, thanks to vinyl. At first, there was no time limit for church music and court music (Beethoven’s Ninth Symphony lasted about 70 minutes), but as music can be stored, copied and distributed by new technology, a piece of music is also "framed" in the poor capacity of 4 minutes when vinyl was born. Musicians can choose not to compromise on technology, but as long as you want to seize the dividend of new technology, you must make the work shorter and make the melody and rhythm more accurate to meet the increasingly impatient attention of the audience.

Well, whether you like it or not, this process is repeating itself. The emergence of new technical media such as bytes makes human attention shorter than before, which also makes the time of a song shorter than before.

Maybe a hundred years later, the new generation of young people will look back at Jay Chou in the 21st century, just as we look back at Beethoven in the 18th century-"It’s just a song, why did you write it so long?"

Author: Li Beichen (WeChat: Whale Fall Business Review)

?　　Foreword　　

In 1993, Belgian scientists discovered a new antibody-nano-antibody (VHHs) in camel serum, which is different from the traditional antibody structure and only consists of heavy chain variable regions. Compared with traditional antibodies, nano-antibodies have the advantages of stable physical and chemical properties, low immunogenicity, strong tissue permeability, and can recognize epitopes that traditional antibodies cannot recognize, which makes them a strong and beneficial supplement to traditional antibodies.

Nano-antibody is only composed of heavy chain variable region, with small molecular weight and simple structure, which enables it to realize stable expression in various expression systems. So far, VHHs has been expressed in many expression systems, from prokaryotic cells, yeast, fungi, insect cells, mammalian cell lines to plants. This paper mainly introduces the expression of nano-antibody in different expression systems, and the present situation and development trend in the field of this system.

Prokaryotic expression system　Prokaryotic expression system is the earliest mature protein expression system studied. Obtaining foreign proteins through prokaryotes can obtain gene expression products in a short time, and the cost required is relatively low. The expression system mainly includes Escherichia coli expression, Bacillus subtilis expression and Streptomyces expression. Among them, Escherichia coli system is the most widely used, and the prokaryotic system generally refers to the expression of Escherichia coli.

Escherichia coli expression system　Escherichia coli expression system is the most important expression platform of recombinant protein in laboratory environment, and the expression is controlled by lactose operon. The host of Escherichia coli system has the advantages of fast growth, simple culture, low price, clear genetic background and high protein expression. However, it can not modify the expression product after translation, and the expression product contains a lot of endotoxin and is easy to form inclusion bodies.

Common host bacteria of Escherichia coli expression system

Periplasmic space, as the only compartment with oxidation environment in Escherichia coli, is the most commonly used expression system of nano-antibodies containing specific disulfide bonds. In addition to aerobic environment, there are many partners and isomerases in the periplasmic space, which can promote the correct formation of disulfide bonds and help protein fold. Nano-antibody protein is first produced in the cytoplasmic space, and then secreted into the periplasmic space through Sec pathway, SPR pathway or Tat pathway. After signal peptide is removed by signal peptidase, the target protein can be obtained [1].

Up to now, many VHHs have been successfully expressed in periplasm, some of which can reach tens of milligrams per liter, but there are still a lot of VHHs with low yield. This is because many fusion proteins can not be effectively secreted in the periplasmic space, and cytoplasmic expression seems to be the only expression mode of such fusion proteins [8].

The cytoplasm is a reduced compartment, so that the proteins expressed through the cytoplasm can not be folded correctly, and most of them are inclusion body proteins. The proteins are protected from the degradation of intracellular enzymes and are easy to be separated in high purity and concentration. However, there are many kinds of proteins in cytoplasm, and it is difficult to purify them. At the same time, nonfunctional inclusion proteins need to be completely denatured and refolded to be recovered from these inclusions, but the recovery of VHHs is usually inefficient [1]. In order to solve the problem of cytoplasmic expression, Xue X and others designed a new dialysis-dilution device in 2016. Using this device, researchers refolded VHHs and achieved a recovery rate of 85%, while the conventional dialysis and dilution methods only reached 13%[9]. Bacterial cytoplasm can be an effective compartment to produce correctly folded nanoparticles when expressed by recombinant vectors with sulfhydryl oxidase and disulfide isomerase activities. Larger volume and wider cytoplasm of chaperone/folding enzyme can achieve high yield of nano-antibody [6]. At the same time, double mutants with defects in thioredoxin and glutathione pathways have been developed. Some of these strains have been sold commercially in recent years, such as Origami (Novagen), Rosetta-gami (Novagen) and SHuffle?T7 (New England Biolabs) [10]. Among them, Shufflet 7 overexpresses a critical periplasmic isomerase DsbC, which can effectively catalyze the isomerization of disulfide bonds. Some studies have found that,The activity of isomerase DsbC can increase the yield of functional recombinant protein in periplasmic research [11].　　

Kavousipour Soudabeh and other modified vectors added four different signal peptide sequences to guide the secretion and expression of nano-antibodies, and found that TrBC signal peptide had the best effect [5]. Jianli Yu et al. used repetitive amino acid sequence motifs (GAGAGS) found in silk fibroin (SFP) as a new fusion tag (SF-tag) to enhance the expression of nanosomes in E.coli.. The transcription level of labeled protein is 2.3-7.0 times that of unlabeled protein, and the yield is increased by 2.5-7.1 times [27]. Xi Xie and others tried to select antifreeze protein AXX, and obtained its reverse transcription protein XXA by synthesizing XXA gene, which was used to develop new soluble fusion tags. It was found that the solubility ratio of fusion protein expressed by XXA fusion tag reached 86%, which was much higher than that of other tags [12].

Other prokaryotic expression systems　Lactic acid bacteria are the most common intestinal probiotics, which are generally considered to be harmless to human body and are very suitable for the production host of therapeutic molecules. However, the existing expression data show that the yield of VHHS produced by lactic acid bacteria is low, about 1-3mg/L, which is much lower than that of Escherichia coli [13,14]. Lactobacillus rhamnosus DSM14870, as a normal flora in human vagina, can express HIV antibody in the form of soluble or cell wall anchor fixation, and is a suitable expression host for HIV antibody [2]. When Bacillus brevis choshinensis was used for 3L fed-batch fermentation, the yield was as high as 3g/L[15]. This system is easy to manipulate genes, has no endotoxin and shows weak protease activity, so it is a very noteworthy system.

Eukaryotic expression system　

Due to the defects of prokaryotic expression system, such as difficult purification of inclusion body protein and incomplete protein modification, people began to use eukaryotic expression system to study genes. At present, eukaryotic expression systems commonly used in genetic engineering research include yeast expression system, insect cell expression system and mammalian cell expression system.

Yeast expression system　Yeast system has clear genetic background, simple operation, easy genetic operation, perfect eukaryotic protein expression control system and advantages of both prokaryotic and eukaryotic expression systems. Yeast eukaryotic protein expression systems include methanol yeast expression system, Saccharomyces cerevisiae expression system, Schizosaccharomyces expression system and Kluyveromyces expression system [17].

Saccharomyces cerevisiae is the first yeast fermented by human beings, which has no specific virus and low cost. At present, it is the largest yeast expression system in shake flask culture, with the output exceeding 100mg/L[16]. Some amino acids in VHHs can participate in the recruitment of molecular chaperones in endoplasmic reticulum, thus narrowing the difference of molecular chaperones between Saccharomyces cerevisiae and mammalian cells, which is beneficial to the folding kinetics of nano-antibodies and improving the yield of nano-antibodies in Saccharomyces cerevisiae [17]. The protein expressed by Saccharomyces cerevisiae is prone to hyperglycosylation, and a large number of endogenous proteins are produced during fermentation, so the secretion efficiency of the target protein is low [1]. Considering the above problems, Saccharomyces cerevisiae is gradually replaced by methanol yeast represented by Pichia pastoris.

Pichia pastoris expression system is an efficient expression system of foreign proteins. This microorganism does not accumulate toxic ethanol, but only secretes a few endogenous proteins, and its glycosylation degree is the same as that of mammalian cells, so its immunogenicity is weak, so it is the most commonly used eukaryotic expression system. Pichia pastoris has a strictly regulated methanol inducible AOX1 promoter, which is suitable for high expression of foreign proteins [1]. But methanol is a highly toxic and dangerous chemical product. The GAP promoter without methanol induction is used instead of AXOI, and the carbon source does not need to be changed during the culture process, so the operation is simpler and the time for foreign proteins to reach the peak level can be shortened. The yield and affinity of nanoparticles produced by this system are similar to those produced by methanol-induced expression. It has been studied that the yield of nano-antibody expressed by this system in shake flask is 51.71mg/L[18] and 16mg/L[19].

Fungal expression system　

Some filamentous fungi, such as Trichoderma and Aspergillus, can secrete a large number of foreign proteins in the culture medium. However, the fungal system not only expresses protein of interest, but also produces many proteases, which affects the yield. Joosten et al. obtained only 1.5 mg/L VHHs in Aspergillus awamori medium. After adding 0.25% BSA, it reached 7 mg/L. It is reported that more than 80% of VHH is degraded or adhered to the cell wall compared with endogenous xylanase secreted efficiently in this system, and researchers believe that the low secretion efficiency of VHHs is the bottleneck of fungal expression [20]. In order to overcome this limitation and protect VHHs from degradation, other researchers fused VHH with the high amylase A signal sequence (sTAA) of Rhizopus oryzae lipase (N28) and the N-terminal 28 amino acid fragment (N28), so that the highest yield of VHHs expressed in Aspergillus oryzae reached 73mg/L, which was 300-800 mg/L lower than that of Escherichia coli [21]. The final yield of GlaB-VHH fusion protein in Aspergillus oryzae can reach 610mg/L, which provides the possibility for high expression of nano-antibody, but its stability needs to be verified [22]. In addition, VHH[23] was expressed by maize head smut in unconventional secretion pathway.

Insect cell expression system　

Insect cells are a widely used eukaryotic expression system, and recombinant baculovirus vectors (such as AcMNPV) can specifically transfect insect cells. Its survival in nature is short, and the strong promoter is inactivated in mammals and cannot infect vertebrates, so the recombinant baculovirus is safe for human beings. Proteins with complex structures under the control of strong promoters may not be able to form correct folding and post-translation modification, which may lead to the aggregation of target proteins and reduce antibody production, so the expression yield of VHHs in this system is much higher than that of other types of antibodies [17].

Gómez-Sebastián and others used the improved baculovirus expression system (IBES? technology) to express VHHs for the first time. The chigger larvae were inoculated with different recombinant baculovirus inoculation methods. The yield of VHH extracted from larvae is 257 mg/L, and the recovery rate after purification is 50%[7]. When expressing anti-endothelial cell selection protein, Drosophila cell SC-2 secreted 12-14mg/L, but the target protein was not detected when expressed in Escherichia coli at the same time, which may be related to the processing and modification ability of insect cells [26].

Mammalian cell expression system　Mammalian cells are the best expression system for therapeutic antibodies, because the produced antibodies have the weakest immunogenicity. At present, the main cell lines include CHO, NS0, Sp2/0, HEK293 and per C6 [17]. Among them, CHO cells grow rapidly and adapt to serum-free suspension culture, which is the most popular host. They can express VHHS up to 100mg/L in shake flask [24]. HEK293 cells are more commonly used in the laboratory because of transient transfection and semi-stable expression, because they require less time and labor. However, monoclonal antibodies approved for the treatment of human diseases are only produced by CHO, NS0 and SP2/0. So far, HEK293 cells have not been used to produce VHHs, and there are few reports about producing antibody fragments by this method [17]. In most literatures, CHO is used to produce antibody fragments. Up to now, 50% of the therapeutic proteins produced in industry are still produced by CHO [1]. The production costs associated with mammalian cell lines are much higher than those associated with any other laboratory research platform.

Common host cells of mammalian expression system

Plant expression system　Compared with other expression systems, plant production platform (stable and transient expression system) is cheap, safe, capable of folding and assembling complex proteins and post-translation modification, easy to expand, and unaffected by human pathogens and diseases. It is an ideal protein expression host and is usually used as an alternative method for mammalian expression systems. In addition, plant expression products can be concentrated in specific organs, such as leaves or seeds, and can be taken orally directly. Tobacco leaves are the most common hosts for expressing VHHs, while the proteins expressed in Arabidopsis and rice seeds have attracted much attention because of their direct oral input of VHHs[1]. The stable transformation methods commonly used in plant system expression have some problems, such as the recombinant gene escaping to nature or the time-consuming transformation. Therefore, some studies have proposed the transient expression system of plant virus expression vector as an alternative method, and the nano-body content with correct conformation reaches 0.45% of the total soluble protein [25].

Present situation and development of nano-antibody expression　

Escherichia coli expression system is the most commonly used expression system of nano-antibody, which has two expression modes: periplasmic expression and cytoplasmic expression, each with its own advantages and disadvantages. Some oxidation engineering strains have been modified, but more experimental support is needed. In addition, various methods of modifying vectors, such as adding labels or signal peptides, have been put forward and have been verified by experiments. Compared with Escherichia coli, Pichia pastoris has excessive glycosylation, which may lead to immunogenicity and ultimately affect its clinical application. The expression systems of fungi, insects, plants and mammals are much more complicated than prokaryotic and eukaryotic expression systems, and are generally not easily adopted after considering the production time, cost and laboratory conditions.

Escherichia coli system is fast, convenient and cheap, and it is the first choice for VHH expression under most laboratory conditions. When some types of VHHs can’t be successfully produced in E.coli, other systems will be used instead.

List of nano-antibody expression

　　When it comes to cancer, our first impression is often a terminal illness, which makes people "talk about cancer." The data shared at the 10th annual meeting of the National Cancer Center shows that the 5-year survival rate of cancer in China has increased from 30.9% to 40.5% in the past decade. Although the incidence of cancer has increased, with the change of public concept and the progress of medical treatment, cancer has become a chronic disease in China. ①②

　　Figure from White Deer Video ②

　　In fact, it may take several years from early cancer to late cancer. And with the development of medical technology, after scientific treatment, the five-year survival rate of many cancers has been above 90%.

　　Cancer has become a chronic disease.

　　The "Core Information and Knowledge Points of Cancer Prevention and Control" issued by the CDC of the National Health and Wellness Commission pointed out: "Cancer is a kind of chronic disease that seriously endangers people’s health." ③

　　It may take several years for cancer from early stage to late stage.

　　The development of most cancers is not so fast, and the pathogenic process of cancer is also very long. It takes quite a long time from normal cells to cancer cells, from continuous division and proliferation into cell clusters to "visible" diagnosable cancer nodules, ranging from a few years, usually taking more than ten to twenty years, or decades. ④

　　It’s just that some symptoms are ignored by us as ordinary physical discomfort, so that once they are discovered, they are in the late stage. Naturally, they feel that the disease is progressing rapidly and it is difficult to treat and recover.

　　Figure from Shanghai CDC ⑤

　　2. Scientifically treat some cancers, and the five-year survival rate is over 90%

　　With the change of people’s concept and the progress of medical level, cancer has changed towards the development direction of chronic diseases in China. In 2021, according to the statistics of the National Cancer Center, the five-year survival rate of malignant tumors has increased from 30.9% ten years ago to 40.5% at present, an increase of nearly 10 percentage points. For some malignant tumors, such as esophageal cancer, the five-year survival rate has been higher than that of developed countries such as the United States. ⑥

　　Yao Zhendan, deputy chief physician of the Fourth Ward of Gastrointestinal Cancer Center of Peking University Cancer Hospital, said in a video interview with Bailu that the treatment effect of early cancer is excellent. According to statistics, the five-year survival rate of early gastric cancer and intestinal cancer is above 90%, the five-year survival rate of middle gastric cancer is about 50%, and the five-year survival rate of middle intestinal cancer is about 70%. Even for patients with advanced cancer, the survival rate becomes better after treatment, and the survival time with cancer is longer. ②

　　Image from White Deer Video

　　Therefore, early detection and treatment of cancer is very necessary.

　　15 small signs of pre-cancer to know!

　　What are the symptoms and signs of early cancer? This is a problem that many people are concerned about. Pan Zhanhe, deputy chief physician of the Department of Oncology, Zhongshan Hospital affiliated to Xiamen University, listed 15 possible symptoms and signs in the early stage of cancer in the "Oncologist" WeChat publication in 2018 for your reference. ⑦

　　1. Masses and nodules

　　Mass nodules are not necessarily cancer, we can make a preliminary judgment on it:

　　Look at the size. The smaller the nodule, the more likely it is to be benign, of course, it is not absolute, so we should pay attention to follow-up and review; Pay attention if the nodule is more than 1 cm. Note that nodules that continue to increase, especially those that increase rapidly, tend to be malignant and should be paid special attention to.

　　Look at the texture. Soft or cystic masses tend to be benign; Hard, fixed or solid masses tend to be malignant, but the swollen lymph nodes of lymphoma are tough to the touch.

　　Third, look at the boundary and activity. Benign tumors usually have clear boundaries and good activity; Most malignant tumors have unclear boundaries, often adhere to or fuse with surrounding tissues, and their activity is poor.

　　Health Times Mao Yuanyuan/photo

　　2. Persistent pain is not traumatic

　　If there is persistent pain in a certain part of the body that is not caused by trauma and has unknown reasons, you should go to the hospital to find out the reason.

　　3. Cough if you don’t cure for a long time

　　For chronic cough or blood in sputum, especially long-term smokers, we should be alert to the possibility of lung cancer.

　　4. Unexplained total fever

　　If the cause of fever is unknown and persists, you should be alert to the possibility of cancer and conduct relevant examinations as soon as possible.

　　5. Inexplicable emaciation and fatigue

　　If you don’t take the initiative to lose weight, you don’t deliberately go on a diet, but you lose weight for unknown reasons, or you are accompanied by anorexia, fatigue, fatigue, etc. To find out the reasons, emaciation and fatigue may also be one of the manifestations of cancer.

　　6. No trauma but bleeding

　　Non-traumatic abnormal bleeding, such as hematochezia or hematemesis, should pay attention to exclude the possibility of intestinal cancer and gastric cancer; Beware of lung cancer when coughing up blood and taking blood in sputum; Irregular vaginal bleeding or menstrual bleeding may be related to gynecological tumors such as endometrial cancer and ovarian cancer. Painless hematuria or dysuria, beware of urinary tumors; Non-traumatic epistaxis should be careful to rule out nasopharyngeal carcinoma.

　　7. Abnormal digestion persists.

　　If indigestion persists, or accompanied by acid reflux, heartburn, abdominal pain and discomfort, we should be alert to the possibility of gastric cancer or esophageal cancer.

　　8. Obstruction of swallowing food

　　If you have a feeling of bloating or burning behind the sternum, pain behind the sternum, or a feeling of foreign body in the esophagus, especially the feeling of swallowing food obstruction, you should be alert to the possibility of esophageal cancer and laryngeal cancer.

　　9. hoarseness persists and is difficult to recover

　　If hoarseness is difficult to recover and lasts for a long time, we should be alert to the possibility of laryngeal cancer, lung cancer and so on invading or oppressing the recurrent laryngeal nerve.

　　10. Changes in stool habits and characteristics

　　If the habit, frequency or character of stool changes (such as thin stool shape, pus in stool, blood and mucus, etc.), or constipation and diarrhea alternate, be alert to colorectal cancer and go to the hospital for examination as soon as possible.

　　11. White spots increase burning and itching

　　Oral leukoplakia, leukoplakia of female vulva or male penis glans rapidly expand with burning itching, and attention should be paid to eliminating canceration.

　　12. Mole enlargement and discoloration

　　If the mole suddenly increases, the edge becomes irregular or the color changes, or there is bleeding or burning pain, and the original hair on the mole falls off, it is suspected that melanoma may be possible.

　　13. Fracture is easy to occur.

　　Fractures that are easy to occur without obvious or slight external force may be pathological fractures, so we should be alert to bone malignant tumors. Teenagers should pay attention to leukemia if they have non-traumatic joint pain and swelling.

　　14. Ulcer wounds persist for a long time

　　If the skin wound or ulcer persists, be alert to the possibility of skin cancer. Oral ulcer is very common, but if it cannot heal for a long time, we should be alert to the possibility of oral cancer.

　　15. Abnormal changes in male breasts

　　Although male breast cancer is rare, if male breast grows abnormally or has other abnormal changes, you should be alert to the possibility of male breast cancer and go to a breast specialist as soon as possible.

　　Of course, these manifestations are not necessarily cancer, but remind you to pay attention to it and go to the hospital for further examination to make a clear diagnosis.

　　Early and timely detection,

　　Kill cancer in the cradle

　　For ordinary people, regular physical examination can keep abreast of their physical health; Especially in the age of high incidence of cancer, timely screening can minimize the risk. Recommendations for screening and prevention of common malignant tumors in residents (2023 edition)
It is suggested that ordinary people with general risks can arrange screening in this way: ⑧

　　Health times chart

　　For high-risk groups, especially those with a family history of cancer, screening and physical examination should be done in advance and regularly according to the doctor’s advice.

　

　　This article is synthesized from:

　　① On March 29th, 2023, Shanxi Daily held the 10th Annual Academic Conference of National Cancer Center.

　　② 2023-08-14 White Deer Video "# Cancer has become a chronic disease in China #"

　　③ Key Information and Knowledge Points of Cancer Prevention and Control by CDC of National Health and Wellness Commission.

　　④ 2020-09-29 WeChat WeChat official account "Oncologist" "Who is easy to get cancer? Doctors say that people with cancer belong to these five categories! 》

　　⑤ 2020-06-30 Shanghai CDC "Science of Cancer Prevention? Is cancer a chronic disease? 》

　　⑥ Written Record of the Regular Press Conference of the National Health and Wellness Committee on April 27th, 2021.

　　http://www.nhc.gov.cn/xcs/s3574/202104/df811bc1ee8b4c8db0f0561758172ca8.shtml

　　⑦ 2018-10-29 oncologist "The most comprehensive cancer science: cancer prevention, early diagnosis, screening and treatment. The whole article includes "

　　⑧ Recommendations for Screening and Prevention of Common Malignant Tumors among Residents (2023 Edition). Shanghai Anti-Cancer Association, Cancer Hospital Affiliated to Fudan University.

Work hard and move forward bravely.

　　Looking back on the past, people in Guangdong state-owned enterprises, with a sense of urgency that they can’t wait, a sense of crisis that they can’t wait, and a sense of responsibility that they can’t sit still, have made great efforts to grasp the iron mark and step on the stone to leave a seal, and carried out their mission with practical actions.

　　It is they who have built criss-crossed rail transit networks, expressway networks, airport aviation networks, port shipping networks, natural gas pipeline networks and water safety guarantee networks, and undertaken a number of major projects such as the "5+4" airport group, the Guangzhou-Shantou Railway, the Guangzhou-Zhanjiang Railway, the Nansha Bridge and the Shenzhen-China Passage, and promoted the realization of "high-speed railway connecting cities and counties".

　　They are the main force in major livelihood projects such as water resources allocation, comprehensive environmental protection improvement, paddy field reclamation, etc. They have built water resources allocation projects in the Pearl River Delta, eastern Guangdong and Guangdong around Beibu Gulf, benefiting 62 million people, and the comprehensive environmental protection improvement in Lianjiang River Basin has benefited 4.3 million people, completing the reclamation of paddy fields of 118,900 mu;

　　It is they who have laid out a number of major industrial projects in the fields of advanced materials, semiconductors and integrated circuits, green petrochemicals, automobile manufacturing, biomedicine, etc., helping our province to build a more integrated and smooth industrial ecosystem;

　　It was they who took the lead in the fight against the COVID-19 epidemic, completed the R&D and production tasks of mask machines in an unconventional way, reduced the highway toll by over 15 billion yuan, and reduced the rent by over 5 billion yuan;

　　It is they who bravely shoulder heavy burdens in poverty alleviation, rural revitalization, emergency rescue and disaster relief, and stable employment, and fully demonstrate the responsibility of state-owned enterprises in social and people’s livelihood security and social responsibility.

　　The new way to catch up with the exam gives a new mission of the times, and the new struggle journey calls for new responsibility. 2022 is the year of the 20th National Congress of the Communist Party of China, the closing year of the three-year reform of state-owned enterprises, and the opening year of implementing the spirit of the high-quality development conference of state-owned enterprises in the province. The state-owned enterprises in the province have great responsibilities and glorious missions. People in Guangdong state-owned enterprises will be guided by Socialism with Chinese characteristics Thought of the Supreme Leader in the New Era, always keep a mental state of hard work and courage, forge ahead in the new year, and greet the convening of the 20th Party Congress with excellent results!

　　Stepping on the new year’s footsteps, stepping on the drums,Guangdong state-owned enterprises are in front-line positions.To friends from all walks of lifeSend sincere New Year greetings!

Airport group

Baiyun airport

　　Guangzhou Baiyun International Airport, under the jurisdiction of Guangdong Airport Group, is one of the three national international aviation hubs.Baiyun airport operation control centerIt is also the "brain center" of the whole airport operation, mainly responsible for the operation command, apron control, emergency rescue, flight coordination and epidemic prevention and control of Baiyun Airport. On the road of pursuing the operation quality of world-class airports, the operation control center keeps forging ahead courageously, promotes the refined management of flight operations, continuously improves the management level of flight normality, ensures that the punctuality rate of Baiyun Airport is among the highest in the country, and helps Baiyun Airport to strive for a world-class aviation hub. I wish you all the Year of the Tiger: take the tiger’s luck, walk the tiger’s step, live the tiger’s power, show the tiger’s posture, gather the tiger’s spirit and be a tiger!

　　In 2022, the Year of the Tiger was a lucky year, Jin Hu Nafu, and the Spring Festival came.Baiyun airport security inspection security guard departmentMainly responsible for the safety inspection of passengers leaving the terminal and the monitoring of arrival area, flight area and enclosed area. On the occasion of the Lunar New Year, the Compass Team of the Fifth Brigade of the Security Guard Department of Baiyun Airport wishes everyone that in the new year, all hopes can be realized one by one, all dreams can come true one by one, all expectations can come as scheduled, and all efforts can be rewarded!

　　Baiyun airport fire control security centerMainly responsible for the fire protection, fire fighting and rescue of Baiyun Airport and the security of the jurisdiction. Among them, the third fire and security brigade is responsible for the fire monitoring and emergency treatment of the first fire in Terminal 2. During the Spring Festival, all the staff of the brigade still stick to their posts and dedicate themselves to serving the public and guarding the travel safety of the majority of passengers. On the occasion of the Spring Festival, the Thunder Team of the Third Fire Brigade wishes everyone a new atmosphere in the New Year and a prosperous Year of the Tiger.

Jieyang Chaoshan airport

　　Chaoshan is a famous hometown of overseas Chinese, bordering Fujian Province in the east, Shanwei City in the west, South China Sea in the south and Meizhou City in the north.Jieyang Chaoshan airport safety inspection officeMainly responsible for the safety inspection of passengers and their luggage, air cargo and mail aboard civil aircraft. The security checkpoint adheres to the team culture of "innovation-driven, ingenuity-built, and pursuit of Excellence", pays close attention to epidemic prevention and control, builds a safe defense line, and practices sincere service. During the Spring Festival this year, all the staff at the security checkpoint worked with one heart and one mind, stuck to their posts and moved forward bravely. On the occasion of the Spring Festival, the Chaoshan security inspector sent a New Year greeting: I wish you all a lucky and prosperous Year of the Tiger!

huizhou pingtan airport

　　Huizhou airport ground service departmentMainly responsible for check-in stowage, passenger inquiries, passenger guidance, etc., adhering to the concept of "safe development, sincere service", with sincere feelings, we provide every bit of warm-hearted service and sincere warm care for travelers. The service measures are precise and accurate, and the service system is improving day by day. In 2022, the ground service department will continue to work hard, persevere and be full of enthusiasm and hope to jointly write a new chapter of high-quality development. At the beginning of the new year, the Year of the Tiger is approaching. The ground service department of Huizhou Airport wishes you a happy New Year!

Shaoguan airport

　　Shaoguan Airport is a "recruit" of Guangdong Airport Group.Shaoguan airport security inspection fire protection guard departmentShoulder the fire protection and fire monitoring work within the airport to prevent fire accidents. The fire protection department of security inspection adheres to the principle of "zero tolerance for potential safety hazards", firmly believes in navigation, and takes the initiative to successfully complete the navigation fire protection work. In the new year, the security and fire protection department wishes all passengers a safe trip and a smooth return home!

Guangdong environmental protection group

Guangye environmental protection

　　Shantou Beizhou Sewage Treatment PlantIt is a key pollution reduction project in Guangdong Province during the 11th Five-Year Plan, with a construction scale of 120,000 tons/day, and adopts the improved A2/O oxidation ditch production +MBBR+ magnetic coagulation sedimentation sewage treatment process. On the occasion of the Spring Festival, Guangdong environmentalists who stick to the sewage treatment site wish you all the best, smooth sailing and good year after year.

Guangdong hongda

　　Guangdong hongda Pakistan projects departmentLocated in thar coalfield, thar desert, southeast of Sindh province, Pakistan, thar coalfield is the largest coal area discovered in Pakistan so far, and the seventh largest brown coal mine in the world and the largest in Asia. The second block project in thar coalfield is the key project of the Belt and Road Initiative, the benchmark project of the China-Pakistan Economic Corridor and the first coal-electricity integration project in Pakistan. Since the project was launched in 2016, Guangdong Hongda has undertaken part of the first phase of the mine construction stripping project, and now it undertakes part of the first phase stripping operation and maintenance project and the second phase stripping coal mining project. During the Spring Festival this year, 54 Chinese employees and 792 Pakistani employees of the Project Department kept to their posts. On the occasion of the arrival of the Spring Festival, the grand people who struggled in thar desert, Pakistan, sent a fiery New Year greeting: I wish you all the best in the Year of the Tiger and all the best!

Guangsheng group

Zhongjinling south

　　Trolley blasting class in mechanized mining workshop of Fankou lead-zinc mine in Zhongjin Lingnan.It is a veritable mechanized operation team, with 12 employees and 21 operation trolleys, mainly engaged in the tasks of rock drilling and blasting in each middle section of the underground. With the slogan of "enterprise development, I am the pioneer", combined with activities such as "safety standardization and post compliance" and "strengthening cost control implementation plan", they have exceeded various safety production tasks for many years in a row. In 2021, the blasting class of mechanical mining trolley was awarded the honorary title of "National Worker Pioneer" by the All-China Federation of Trade Unions for its outstanding performance. On the occasion of the Spring Festival, the employees of the machine-mining trolley blasting class who fought in the deep underground Sh-320m second section 32# orebody stope sent a New Year greeting: I wish you all a vigorous year of the Tiger! Happy family! Peace and good luck!

Guo xingguang Dian

　　As the main force in the electronic information sector of Guangsheng Group, Guoxin Optoelectronics has always been committed to serving the national strategy, supporting national construction and leading the development of the industry. It has continued to promote the development of the ultra-high-definition industrial ecological chain and launched an ultra-high-definition backlight solution that integrates cutting-edge technologies such as 5G and 8K, empowering thousands of display scenes with the road of scientific and technological innovation, and presenting people with an ultra-high-definition feast. In order to speed up the mass production process of ultra-high definition Mini LED backlight new products and promote the commercialization of Mini LED,Guo Xingguang Dian Mini LED backlight projectFront-line employees stick to their posts, work hard and strive to win a good start in high-quality development! On the occasion of the arrival of the Spring Festival, the national stars who fought in the front line of the workshop sent the warmest "star" New Year wishes: the Year of the Tiger is prosperous and powerful!

Dongjiang environmental protection

　　Shaoguan Dongjiang Safe Landfill ProjectIt is the second-phase construction project of the hazardous waste treatment and disposal center in northern Guangdong. It was completed at the end of 2020. In 2021, it obtained the Hazardous Waste Business License, approved the treatment of 18 categories of landfill wastes, with an operating scale of 34,500 tons/year. In April, the stabilization and safe landfill started trial operation, and now it has achieved normal operation. During the Spring Festival this year, the employees of the company responded to the national call for epidemic prevention. Most employees chose to spend the New Year on the spot and stick to their posts. On the occasion of the arrival of the Spring Festival, all the colleagues in Shaoguan Dongjiang wish you a happy New Year! Happy family!

Guangdong energy group

Zhanjiang electric power company

　　Zhanjiang electric power company boiler ontology maintenance projects departmentWith the work style of "strict, meticulous and practical" and adhering to the team spirit of sincere cooperation, we have successfully completed the boiler overhaul of four 330 MW generator sets in our factory, fully ensuring safe production and timely delivering safe and reliable electric energy for social development. "Don’t relax during holidays" is their self-requirement to stick to their posts and do their jobs well during holidays, and it is also a promise to implement safety production and ensure power supply. On the occasion of the Spring Festival, all members of the project department wish you all the best in the year of the tiger! Fu Hu cheng Xiang everything is happy!

Jinghai power generation company

　　Jinghai Power Generation Company, located on the Jinghai Bay in Jieyang, Guangdong, is one of the super-large backbone enterprises of Guangdong Energy Group. This spring festival,Jinghai power generation company equipment departmentOur young employees will stick to their posts to ensure power supply, insist on interpreting "labor is the most glorious" with hard work and sweat in ordinary posts, go all out to do a good job in safety production, ensure the safe and reliable operation of generator sets and equipment, and protect the lights of thousands of households with practical actions. On the occasion of the Spring Festival, I wish you all a happy New Year! Good luck in the year of the tiger!

Bohe energy company

　　Bohe energy companyLocated in Electric Town, Binhai New Area, Maoming City, Guangdong Province, it has two 1,000 MW ultra-supercritical coal-fired generating units put into operation in 2020. The units adopt "ultra-low emission" environmental protection technology, and simultaneously build environmental protection facilities such as flue gas desulfurization, denitrification and wastewater recovery and comprehensive utilization. It is a beautiful modern coastal power plant in the Beibu Gulf urban agglomeration area of western Guangdong. During the Spring Festival of the Year of the Tiger, the front-line employees of the company will stick to their posts and continue to fight to ensure the continuous delivery of safe and reliable electric energy to the society. On the occasion of the arrival of the Spring Festival, I sincerely send my New Year greetings to marina bay. I wish you all happiness and well-being.

shajiao a power plant

　　shajiao a power plantFocusing on the new development concept, we will focus on the strategic planning of clean energy development, accelerate the transformation and upgrading of energy structure, and fully promote the construction of local alternative power supply projects and the safe and stable development of in-service units. This spring, most employees of this factory, especially the young employees born in 1998, chose to stay in Dongguan for the New Year, stick to their duty posts together with the front-line duty personnel, do a good job in ensuring power supply during the Spring Festival and overhauling the units after the holiday, and provide safe and efficient power supply and energy guarantee for the economic and social development of Dongguan and the construction of marina bay New District with practical actions. Here, I wish you all good luck in the Year of the Tiger! Peace and joy! Add wings to the tiger!

Communications group

Sheng highway construction company

　　During the Spring Festival,Guangzhou-Shenzhen Expressway organizes volunteers.Carry out convenience services at service points along the route, and provide hot tea, emergency food, route guidance, handling ETC anomalies and other services for drivers and passengers. They said: "Although we can’t spend the holidays with our families, it is worthwhile to serve more drivers and passengers in our posts! I wish you all a happy new year, good health and all the best! "

　　Jiangluo Expressway Dongcheng Central StationResponsible for the daily management of three toll stations: Dongcheng, Xinxing and Rencun. During major holidays, the traffic flow to and from Xinxing County, the hometown of six ancestors, is like weaving. In order to ensure the safety and smoothness of the station, during the Spring Festival this year, most of the toll station staff still stay at their posts and spend the New Year in the same place. I only hope that the majority of drivers will "sit in a happy car, go through the door as they wish, shake the safety wheel, walk along the harmonious road, return home happily and walk with each other happily!"

　　"women soldiers" in Class B of Tonghu Toll Station of Boshen ExpresswayFull of sunshine, colorful and full of vitality, in the three-foot booth, they used their efforts and struggles to shed their youth and sweat, and sent New Year greetings to the past passengers in the toll booth square: the first ray of sunshine in the morning is our blessing, and the last ray of sunset is our greeting. I wish you good health, all the best and prosperity!

United electric service company

　　United electric service companyResponsible for the implementation of Guangdong expressway network toll collection and the promotion of Yuetong card electronic toll collection (ETC). Adhering to the corporate philosophy of "service, innovation and high efficiency", the company closely focuses on the strategic positioning of "five centers", relies on the services of split settlement of online charges and ETC issuance, implements the development concept of "coordination of people, vehicles and roads", creates a new era of digital empowerment of the Group, and provides users with more efficient, safer and better travel services. On the occasion of the Year of the Tiger, United Electric Service Company wishes everyone a happy New Year and all the best!

Yueyun rescue company

　　Yueyun rescue companyResponsible for the rescue business of about 7000 kilometers of expressway, it is the largest professional vehicle rescue enterprise in the province. During their stay in Spring Festival travel rush, all rescuers stood by their posts and stood by 24 hours to protect the safety and smoothness of the expressway and the reunion of thousands of families. Guangdong sports saves all employees. I wish you good luck in the Year of the Tiger, a happy family and a safe journey!

Xin yue Jia fu asphalt company

　　Xin yue Jia fu asphalt companyProduction and supply of "Nanyue" self-owned brand modified asphalt and emulsified asphalt provide a strong guarantee for the Group’s expressway construction and maintenance projects. All colleagues of Xinyue Jiafu wish everyone a happy New Year, a healthy family and all the best!

Port and shipping group

Changqiaogang

　　A trestle, with a history of one hundred years.ChangqiaogangFully tap the location advantages of coastal cities, which are "beautiful fish and shrimp near rivers and seas", and actively plan development, which is glowing with new development vitality. During the Spring Festival, party member Pioneer Team of Changqiao Port took an active role, stuck to its post, did a good job in the prevention and control of normalized epidemic situation, ensured the smooth market order, and delivered fresh and delicious aquatic products from the sea to thousands of households. On the occasion of the Spring Festival, all my colleagues in Changqiao Port wish you a prosperous and prosperous Year of the Tiger!

Red-billed gull cruise ship

　　Red-billed gull harbor tour is a fine line product of coastal tourism in Zhanjiang, which gathers a large number of scenic spots such as Jinsha Bay, sea-watching promenade and Shili military port. It is a harbor landscape integrating military port, fishing port, commercial port and tourist port, and is one of the diamond tourism routes in Zhanjiang. This spring festival,Red-billed gull cruise shipTailor-made service measures for tourists according to local conditions and multiple measures, all staff stick to their posts, do a good job in epidemic prevention and control, and guide cruise ships to swim safely in the harbor. Red-billed gull cruise ship sends new year wishes to everyone: I wish you a happy new year and a good year of the tiger.

New ferry company

　　New ferry companyIt is a local ferry company in Hong Kong acquired and controlled by Hong Kong Shipping Group in 2020. During the Spring Festival, all the staff of First Ferry stick to it to ensure passengers’ safe and smooth travel. When the Spring Festival comes, they are of one mind and wish everyone a happy New Year!

Yuexing shipping

　　Yuexing shippingIt is a subsidiary of Pearl River Shipping, a Hong Kong-based enterprise of Hong Kong Shipping Group. As a shipyard that can provide 24-hour maintenance services for ships in Guangdong, Hong Kong and Macao in Guangdong-Hong Kong-Macao Greater Bay Area, the first-line ship maintenance workers are still busy when the Spring Festival comes. They are on the banks of Xiangjiang River, sending you New Year wishes!

Guangwu holding group

Juzhengyuan

　　Juzhengyuan 1.2 million tons/year propane dehydrogenation to polypropylene projectLocated in Lishadao Fine Chemical Industry Park, shatian town City, Dongguan City, the first phase of the project was officially put into production in October 2019, with an annual production capacity of 600,000 tons of polypropylene. During the epidemic, the medical protective meltblown cloth was converted in time, accounting for 40% of the Guangdong market, providing raw materials for the production of downstream medical protective products in time. The project produces more than 30,000 tons of hydrogen by-product every year, which ensures the price and supply and helps the development of hydrogen energy industry. On the occasion of the Spring Festival, all the staff of Juzhengyuan wish you a happy Spring Festival, like a tiger adding "100 million", a healthy family and all the best!

Guangwu zhongnan building materials

　　Guangwu zhongnan building materialsIt is a large state-owned group enterprise jointly invested and established by Guangwu Holding Group, Baowu Group Zhongnan Iron and Steel Co., Ltd. and Ouye Yunshang Co., Ltd. The company will devote itself to becoming a nationwide influential overall solution service provider of commodity supply chain, and realize the maximum customer value, shareholder value, employee value and social value. The kind cow wagged its tail and went away suddenly, and the blessing tiger offered Rui to the house. On the occasion of the upcoming Spring Festival, I wish you all good luck in the Year of the Tiger in 2022, full of strength and happiness!

Guangwu logistics

　　Guangwu logistics belongs toDongjiang companyMainly engaged in Guangzhou-Guangzhou material terminal, the mode of domestic and foreign trade containers running concurrently, giving full play to the advantages of resources and policies, and constantly improving the management level and service quality by doing a good job in digital construction, extending the logistics service chain and expanding value-added services, so as to provide customers with better and more convenient port services. During the Spring Festival this year, employees of Dongjiang Company will continue to stick to the front line to welcome the arrival of ships from all over the world. On the occasion of the Spring Festival, Guangwu Logistics, together with all employees of Dongjiang Company, sends you a New Year greeting: I wish you all a prosperous and prosperous New Year!

Guangwu automobile trade

　　After-sales service is the guarantee of the whole company’s service.Jiujiang hengming branch after-sales teamQuality-oriented, customer service as the purpose, in the past 2021, all the staff worked hard for the same goal. Hard work and struggle are the only way to success. In 2022, Hengming will be ready to start a new journey. After-sales team wishes everyone: the Year of the Tiger is prosperous, life is booming, career is roaring, and money is thriving!

construction engineering group

Construction engineering group zong contracting business division

　　Guangzhou International Cultural Center ProjectLocated in Pazhou, Haizhu District, Guangzhou, with a total construction area of 161,870.8 ㎡, total building height (tower crown) of 320m, 54 floors above ground and 5 floors underground, the project will become another landmark building with high recognition in Guangzhou, and it will be a key construction project and a "benchmarking project" made by Construction Engineering Group. On the occasion of the Spring Festival in 2022, all the staff of the Guangzhou International Cultural Center project of the General Contracting Division sent sincere New Year wishes: the Year of the Tiger will be blessed and Qi Xin will be prosperous!

Guangdong hydropower

　　Water supply project from Han to RaoIt is a provincial key livelihood project to ensure the water safety of 620,000 Raoping people. After the project is completed, the water transfer scale will be 400,000 tons/day, effectively alleviating the water shortage problem in the core areas of Chaozhou Port Economic Development Zone and Fujian-Guangdong Economic Cooperation Zone, especially in coastal towns. In order to let Raoping people drink Hanjiang River water as soon as possible, more than 100 front-line construction workers of Hanjirao water supply project were held to their posts during the Spring Festival this year. On the occasion of the Spring Festival, Guangdong Hydropower people wish you all the best in the new year: tiger and tiger, good luck and good luck!

　　Niulu Reservoir Project in GuangzhouLocated in liang kou, Conghua District, it is a medium-sized reservoir that mainly supplies water, taking into account flood control, power generation and ecology. This project belongs to the key project of the "Thirteenth Five-Year Plan" of Guangdong Province and is included in the key projects of Guangdong Province and Guangzhou City in 2018 and 2019. After completion, it will further optimize the water resources allocation and water supply pattern in Guangzhou, which is conducive to optimizing the water resources allocation in Liuxi River Basin and improving the flood control system of Liuxi River. On the occasion of the Spring Festival, the project builders sent New Year greetings: I wish you all a happy New Year, good health and a happy family!

Sanjiang company

　　Sanjiang water system connectivity projectIt is an important decision made by the provincial party Committee and the provincial government to improve the ecological civilization in eastern Guangdong. In 2021, all employees will overcome difficulties and work hard, and the Sanjiang water system connectivity project will be basically completed. On the occasion of the coming of the Spring Festival in 2022, the employees who fought in the front line of the project wish everyone a happy New Year and a prosperous life!

Guangxin group

Xingfa aluminum

　　Mold design and manufacturing is the key technology of aluminum profile production, which directly affects the quality and production cost of products. With exquisite polishing technology, it helps the company’s high-quality development. On the occasion of bidding farewell to the old and welcoming the new,Xingfa aluminum Foshan sanshui branch mold business divisionI wish you all: good health, great leaps and bounds, and good luck in the Year of the Tiger!

Weida photoelectricity

　　Weida Optoelectronics, located in Sanshui District, Foshan City, Guangdong Province, is an invisible champion enterprise in Foshan’s manufacturing industry. It mainly produces polarizers for liquid crystal displays. The products have the characteristics of high temperature and high humidity resistance and are widely used in industrial instruments, vehicle-mounted display instruments, medical electronic displays and other fields. Every polarizer is inseparable from the inspector’s insistence on quality, good quality and excellent quality! High quality, expand the market! On the occasion of the Spring Festival in the Year of the Tiger,Weida photoelectricity chengpin inspection teamI wish you all a happy New Year: I wish you all great success and good luck in the new year!

Fosu technology

　　Fosu technology dongfang electrician mo branchFocus on the research and development and production of specialized capacitor films. The products mainly include roughened films and metallized films. Relying on technological innovation and refined management, the company builds an excellent corporate culture, builds a pragmatic and efficient excellent team, and strives to achieve high-end industries and internationalized operations. The products are sold all over the country and in Europe, America, the Middle East and Southeast Asia. On the occasion of the Spring Festival, I wish you all the best in the Year of the Tiger!

Guangqing technology

　　Guangqing technology yayan factoryIt has achieved remarkable results in short construction period, short production arrival time, and profit in the same year of production, realized product structure optimization and upgrading, and laid a solid foundation for high-quality development. This year’s Spring Festival, more than 85% of the company’s key employees outside the province chose to stick to their posts. On the occasion of the Spring Festival, all the employees of the calendering factory who fought in the front line sent a fiery New Year greeting: I wish you all a happy family!

Tourism holding group

Clouds Guest House

　　Clouds Guest HouseAs one of the series of foreign trade projects in Guangzhou in the 1970s, it was completed and opened on June 1st, 1976 to cooperate with the China Import and Export Fair, and mainly received guests such as heads of state and state leaders. The hotel, with a height of 120 meters, was the "tallest building" in China in that year, and was designated as a landmark building by Guangzhou. It has received visitors from trade fairs for 45 years in a row, and it is the only hotel in Guangzhou that has been promoted from Samsung to five stars. During the Spring Festival this year, Baiyun people abandoned the happy time of reuniting with their relatives and stuck to their ordinary front-line posts. They devoted themselves to serving every guest with enthusiasm and smile, so that guests could enjoy the warmth of "home". It’s the Spring Festival, and the Baiyun people who have been accompanying you send you full New Year greetings. I wish you all a happy New Year, a lucky Year of the Tiger and a healthy family!

Landmark Canton Hotel

　　Landmark Canton HotelLocated in Haizhu Square, it has always enjoyed the reputation of "Home of Overseas Chinese". In 2022, the hotel will celebrate its 65th anniversary, forge ahead in the tide of the times, and keep innovating and developing. On the occasion of the Spring Festival in the Year of the Tiger, Huaxia people still stick to their front-line jobs and provide guests with a warm experience like home with a five-hearted service culture of "wholehearted, attentive, sincere, careful and caring". During the Spring Festival, I wish you all the best!

Guangdong Baiyun City Hotel

　　Guangdong Baiyun City HotelIs a three-star tourist business boutique hotel, located in the east of Guangzhou Railway Station Square, a bustling business center, extending in all directions to facilitate your business trip. Jinniu resigns the old year and Ruihu celebrates the new year. Guangdong Baiyun City Hotel will continue to provide you with high-quality services, and wish all colleagues in the society a powerful, prosperous, auspicious and happy new year!

Cts traffic

　　Cts trafficThe main business is road passenger transportation, and the "Concierge No.1" team has undertaken the transportation guarantee work and epidemic prevention emergency transportation guarantee work of previous provincial and municipal conferences. In the new year, CTS traffic people will make greater efforts to advance and pursue Excellence. China Travel Service wishes you all a prosperous, prosperous and powerful New Year!

Yuehai holding group

Pearl river delta water supply co., ltd

　　As a major national water conservancy projectWater resources allocation project in Pearl River Delta, has won the battle of all-round start-up, all-round start-up and all-round excavation, and is entering a new stage of all-round integration. In 2022, tens of thousands of builders will strengthen their confidence, stick to their posts, vigorously inherit the spirit of "model of the times" of the builders of Dongshen Water Supply Project, continue to play the leading role of "stable investment", make unremitting efforts to achieve the goal of opening water ahead of schedule by the end of 2023 and benefit tens of millions of people in the Bay Area, strive to write a new chapter in the water vein of the Bay Area, make every effort to build the water supply lifeline of the Bay Area, and "hard core" will help the high-quality development of water conservancy in Guangdong.

Yuehai real estate

　　In the first year of the Tenth Five-Year Plan,Yuehai real estateShow new achievements, and the business indicators have reached new heights. In 2022, Yuehai Land will further strengthen its confidence and determination, maintain its strategic strength, adhere to the general tone of steady work and strive for progress in stability, and make new achievements and new images in a down-to-earth manner around the main theme of refined and high-quality development!　

Zhongshan yuehai energy

　　Zhongshan yuehai energyIt has two 300,000 kW cogeneration coal-fired units. In the Spring Festival of the Year of the Tiger, Zhongshan Yuehai Energy will continue to adhere to the mission of "dedicating high-quality energy and building a happy home", stick to its post, guard the normal power generation of the unit, and pass on the festive warmth and full blessings to thousands of households!

Yuehai Expressway

　　Yuehai ExpresswayIt is the first BOT expressway operation company in Guangxi Zhuang Autonomous Region, mainly engaged in the project of Xingye-Liujing Expressway in Guangxi. In 2021, the Guigang toll station on this section won the 20th "National Youth Civilization". In the Spring Festival of 2022, more than 160 employees of Yuehai Expressway stuck to their posts, helped the expressway to be safe and smooth, and guarded thousands of families to go home safely!

Yuehai Huamei Bay Hotel

　　Yuehai Huamei Bay HotelLocated in Wan Chai District, Hong Kong, adjacent to the Convention and Exhibition Center, it has been rooted in Hong Kong for many years and is one of the important windows of Yuehai Group in Hong Kong. Since the outbreak of the epidemic, Qi Xin, the hotel staff, has made concerted efforts to overcome difficulties, so as to achieve zero infection in epidemic prevention and control, keep ahead in business competitiveness and not lag behind in social responsibility. In the new year, the hotel will continue to operate steadily and practice the responsibility mission of Chinese-funded enterprises in Hong Kong with practical actions.

Salt industry group

Xuwen Yanchang

　　Xuwen YanchangLocated at the southwest end of Leizhou Peninsula, the seawater in the area meets the standard of Class I seawater and is rich in trace elements, which is known as "golden water quality". This is the largest sea salt production base in Guangdong Province, and the produced "Guangdong Salt" ecological sea salt has won the national ecological origin product protection certification. Every grain of white sea salt is the crystallization of hard work and strict quality control. On the occasion of the Spring Festival, the staff of Xuwen Salt Field sent warm and sincere wishes at the southernmost tip of Chinese mainland: I wish you all a prosperous and prosperous Year of the Tiger!

Guangzhou company

　　Salt, small products, people’s livelihood, heavy responsibility. While firmly ensuring the safety and quality of salt supply, Guangdong salt industry gives full play to the advantages of grass-roots outlets all over the province, and promotes the popularization of safe and healthy salt into communities, villages, schools and restaurants. just asGuangzhou companyLike other employees, the Spring Festival is coming, and the pace of salt popularization among "Guangdong Salt" people in all parts of the province continues. I wish you all good health in the Year of the Tiger! Laugh "salt"!

Tietou group

Group in town bang town fu village task force

　　Shenglong Tea Farm, located in Fengliang Town, Fengshun County, is a key planting base of high mountain selenium-enriched tea.Tietou group in town bang town fu village work duiGive full play to the advantages of resource integration, promote the expansion of tea planting scale, improve quality, and strive to create characteristic agricultural products. Since its arrival, it has invested more than 1.2 million yuan in assistance funds for people’s livelihood projects such as industrial revitalization, consumption assistance and improvement of living environment. On the occasion of the Spring Festival, the working team of Tietou Group in town wishes everyone a happy New Year and all the best! I wish the motherland prosperity and prosperity!

Shanshan railway company

　　Shantou-Shantou Railway, which is led by Tietou Group, is an important part of constructing the high-speed railway passage in the southeast coast of China and an important infrastructure supporting the "Belt and Road Initiative". Among them, Shantou Bay subsea tunnel with a total length of 9,781 meters is the first subsea high-speed railway tunnel with a design speed of 350 kilometers per hour. In the face of high earthquake intensity, high water pressure and high erosion environment, builders have to solve world-class problems such as tunnel structure safety, durability and construction risk. On the occasion of the Spring Festival in the Year of the Tiger,Shantou Bay subsea tunnelAll builders wish you good luck in the Year of the Tiger and a lot of good luck!

Guangzhan railway company

　　Guangzhan railwayIt is a railway project with the highest standard, the longest route and the largest investment in the history of our province, which is led by Tietou Group. It is an important part of China’s coastal railway corridor. After completion, it will greatly shorten the time and space distance between western Guangdong and Guangzhou and the Pearl River Delta region, and will realize the rapid connection between Guangdong-Hong Kong-Macao Greater Bay Area and the west wing of the coastal economic belt, Hainan Free Trade Zone and Beibu Gulf region, which will play an important role in promoting the economic and social development along the line and the coordinated development of the whole province. Last year, the builders of Guangzhou-Zhanjiang Railway worked hard and exceeded their investment tasks. On the occasion of the Year of the Tiger, the first-line builders of Guangzhou-Zhanjiang Railway wish the people of Guangdong a happy New Year.

Hengjian holding company

Shengjianyuan

　　Provincial Construction Institute Structure CenterMainly engaged in the design of architecture, structure and decoration projects, and the representative works are Guangzhou Infinitus Square. In the new year, let’s make concerted efforts to forge ahead, forge ahead with determination and then set out. The structural center wishes everyone good luck in the year of the tiger, and the tiger will show its posture in the year of the tiger. If the tiger comes out of the mountain, the tiger will flourish!

　　Shengjianyuan municipal administration office No.2Mainly engaged in the design of municipal water supply and drainage and solid waste projects. In order to accelerate the development of municipal water supply and drainage in our province during the 14th Five-Year Plan and practice the development concept of "Lucid waters and lush mountains are invaluable assets", we edited the 14th Five-Year Plan for Urban Domestic Sewage Treatment in Guangdong Province, which helped to push the water environment improvement in our province to a new level. At the beginning of the Spring Festival, more than 80% of the employees of the Second Municipal Office stayed behind and stuck to their posts. On the occasion of the Spring Festival, I wish you all a happy New Year, and the Year of the Tiger is full of vigor and vitality!

Nanyue group

Guangdong building materials

　　Guangdong building materialsIt is an enterprise established by Nanyue Group in Saipan, the largest island and capital of the United States Northern Mariana Islands Federation. As the largest local one-stop building materials and home furnishing trading enterprise, Guangdong Building Materials has adopted a business model integrating global procurement, localized operation, one-stop warehousing and logistics and independent financial accounting. It is mainly engaged in the wholesale and retail of building materials and home furnishing products and has been deeply involved in Saipan for more than 30 years. It is deeply loved by local residents and plays an active role in strengthening exchanges and cooperation with Pacific island countries. It is an effective implementation carrier to promote the national "Belt and Road" construction. In the Spring Festival of 2022, all employees of Guangdong Building Materials are still in Do not forget your initiative mind, bearing in mind their mission and working hard at the forefront overseas. On the occasion of the Spring Festival of the Lunar Year of the Tiger, the "Guangdong Building Materials Man" who fought in the front line of Saipan sent a fiery New Year greeting from the tropical islands of the Pacific Ocean: I wish you all good luck in the Year of the Tiger!

Kunlun security company

　　Kunlun security companyIt is an important part of Nanyue Group’s main business of urban comprehensive operation services. The company insists on "grasping quality and building brand" as the center, constantly strengthens project management and team building, and has accumulated a good market reputation in security service projects such as universities, banks, engineering and government units in Macao. Especially since the outbreak, in the face of fierce competition and pressure in the industry, the company has expanded various businesses in various ways while ensuring its market share, and has undertaken security service projects at various ports and units of the Macao Public Security Police Bureau, achieving a steady increase in operating efficiency and a rapid expansion in scale, which has contributed to the Group’s deep-rooted social and people’s livelihood services in Macao. This year’s Spring Festival, the company’s projects are operating normally, and all project service personnel are on duty in rotation. On the occasion of the arrival of the Spring Festival, Kunlun security guards on duty at the front line of Guangdong-Macao customs clearance port wish you good luck in the Year of the Tiger and everything wins!

Lianfeng supermarket

　　Under Nanyue GroupLianfeng supermarket aoda branchLocated in Hengqin Campus of the University of Macau, it is one of 31 Lianfeng supermarket stores under Nanyue Food and Aquatic Products Co., Ltd.. Lianfeng Supermarket specializes in fresh food such as fresh meat, vegetables and fruits, and has always been committed to providing Macao citizens with safe, high-quality, low-cost and convenient fresh food and services. At present, it has become the largest fresh supermarket chain in Australia. This year’s Spring Festival, Lianfeng Supermarket Australia University Store will not close for the holidays, effectively ensuring the daily needs of all teachers and students of Australia University. On the occasion of the festive season, the people of South Guangdong who are fighting in the front line of anti-epidemic protection wish everyone a happy New Year and a thriving life!

Yueke finance group

Guangdong hongtu

　　At the end of the year and the beginning of the year, Yueke Financial Group controlled listed companies.Guangdong hongtuThe first domestic 6800T super-large intelligent die-casting unit, which participated in the research and development, was officially put into production, and the integrated structural parts of new energy vehicle chassis produced by this equipment were successfully rolled off the assembly line, which indicated that the integrated die-casting technology was self-controlled in China. On the occasion of the Spring Festival, the employees of Guangdong Hongtu Die Casting Workshop, who are fighting in the front line of production, send their best wishes: Happy New Year and good luck in the Year of the Tiger!

Guangqing holding group

Craft company

　　Craft companyUndertaking the core business of the Arts and Crafts Section of Guangqing Holding Group, relying on the Provincial Arts and Crafts Treasures Museum, the Provincial Arts and Crafts Research Institute, the Internet platform project (company) and the Guangdong Gongmei Art Expo Center, integrating resources, and striving to deeply tap the treasures of arts and crafts, innovate business models and fully promote industrial transformation and upgrading by giving full play to the advantages of industry experience and influence. On the occasion of the Spring Festival, employees of the craft company bring Lingnan Gongmeiwenchuang to send New Year greetings: I wish you a prosperous Year of the Tiger and a beautiful life!

Guangqing house purchasing

　　Guangqing house purchasing qi Xia gong mei Xiao wan quFully relying on the advantage of being located in Guangfu Commercial and Cultural Center (Yuexiu Dongshankou), Guangqing Cultural Market was established, which combined traditional market with modern creativity to create a life aesthetic space that is deeply loved by young people and integrates shopping and experience. It has been held for 30 times so far. The Spring Festival Cultural Market is one of the highlights. The Guangqing people who planned and organized the event sent sincere New Year wishes to everyone: I wish you all the best!

Trading holding group

Provincial drug exchange center

　　Provincial drug exchange centerAs a provincial-level bidding and purchasing platform for drugs and medical consumables in Guangdong Province, we adhere to the principle of "demand-oriented, price-linked, and market operation", do a good job in online purchasing of drugs and purchasing of drugs and medical consumables by national and provincial groups, and promote the procurement reform of drugs and medical consumables to be at the forefront of the country. In order to ensure the quantity procurement of medical consumables during the Spring Festival, the employees of the company insisted on fighting in front-line positions. The resignation of Taurus is steady, and Tiggo is more energetic! Provincial Drug Exchange Center wishes you good luck in the Year of the Tiger and the spirit of dragon and tiger!

Yueshu network company

　　Yueshu network companyAs the service supporting body of Guangdong’s "Smart State-owned Enterprise" system construction, it undertakes the tasks of state-owned assets supervision sunshine platform research and development, enterprise data service, and cloud network digital intelligence infrastructure construction, and fully promotes the digital transformation of provincial enterprises. In order to ensure the information network security of customers and the group during the Spring Festival, employees of Guangdong Digital Network Company stick to their posts and conduct 24-hour network security patrol duty to ensure the safe and stable operation of the information system. Sail for a bright future and set sail for a new journey in Guangdong. Guangdong Digital Network Company wishes you a happy New Year and a good year of the Tiger!

　　CCTV News:On the morning of December 13th, 2023-2024 China Economic Annual Conference was held in Beijing. At the meeting, Lin Zechang, director of the Comprehensive Department of the Ministry of Finance, said that in order to achieve the goals and tasks of economic and social development in 2024, the proactive fiscal policy in 2024 should be moderately strengthened, improving quality and efficiency, and doing six "efforts".

　　Lin Zechang said that efforts should be made to support the construction of a modern industrial system, actively support scientific and technological innovation, ensure the demand for funds for tackling key core technologies, continue to increase support for basic research, deepen the reform of the allocation and use mechanism of scientific and technological funds, and adjust and optimize the use direction of funds.

　　Efforts will be made to expand effective demand, continue to make good use of local government special bonds and investments in the central budget, give play to the leading role of government investment, make good use of social security transfer payments and other tools, increase residents’ investment through multiple channels, and enhance consumption capacity.

　　Lin Zechang, Director of the Comprehensive Department of the Ministry of Finance:Efforts should be made to promote the coordinated development of urban and rural areas, adhere to the priority development of agriculture and rural areas, and support the comprehensive promotion of rural revitalization. Focusing on ensuring national food security, we will implement the strategy of "storing grain on the ground and storing grain in technology" and steadily implement the subsidy policy for farmland fertility protection.

　　Efforts will be made to promote green and low-carbon development, continue to promote the prevention and control of air pollution and the protection of water ecological environment, increase the protection and restoration of ecosystems, and promote the construction of a large-scale protection system from the top of the mountain to the ocean. Continue to support the green and low-carbon transformation in key industries such as industry, transportation and construction, and continue to optimize the support policies for clean energy.

　　Efforts will be made to safeguard and improve people’s livelihood, continue to implement the employment priority strategy, promote the employment and entrepreneurship of key groups, and strengthen social security measures.

　　Efforts will be made to enhance financial sustainability, strengthen early warning and monitoring of local financial operations, increase tracking and monitoring of areas with weak financial resources and high risks, strengthen financial fund scheduling, and timely discover and dispose of potential risks in financial operations.

　　Doing Five "Big Articles" on Digital Finance of Science and Technology Green and Inclusive Pension

　　At the 2023-2024 China Economic Annual Meeting held by China International Economic Exchange Center, Lu Lei, member of the Party Group and Deputy Director of the State Administration of Foreign Exchange, said that the effect of monetary policy continued to show, and the financial sector was making efforts to do five "big articles" on science and technology, green, inclusive, pension and digital finance.

　　Lu Lei said that the scale of China’s money and credit stock is already very large. It is necessary to deeply understand the connotation and function of activating incremental funds and give full play to the role of idle and inefficient stock funds. Even if the new money and credit grow less, it can also strongly support the development of the real economy. Secondly, with the transformation of economic structure and the return of finance, the investment in credit will be adjusted accordingly.

　　Lu Lei, Deputy Director of the Party Group of the State Administration of Foreign Exchange:In the past, more real estate and local financing platform credit may decline. At the same time, the increment and proportion of loans in key areas such as scientific and technological innovation, manufacturing, green development, and universal benefits are all rising significantly. At the same time, the proportion of direct financing will also increase in the future, which will have a benign substitution effect on loans, and the quality and efficiency of credit services to the real economy will inevitably improve significantly.

　　Lu Lei said that in the next stage, we should actively revitalize the inefficient financial resources and improve the efficiency of capital use. Every year, 80% of all loans in China need to be recovered and put back. By improving the allocation efficiency, the effective revitalization of these stocks and the annual increase can effectively support economic growth.

　　Xinhuanet Beijing, January 12th Recently, a WeChat WeChat official account published an article "Turn to the List of 17 Poisonous Foods Exposed by CCTV and David", which was widely spread on the Internet. It has been verified that the food safety information listed in the article is actually a rumor of malicious tampering or speculation about old news. This rumor is alarmist, creating rumors by means of PS, substituting flowers, pretending to be a fool, and making things out of nothing. The malicious spread has caused some public panic.

　　Rumor makers spread rumors on the internet through careful compilation and editing, which has caused great misleading to the public. Xinhuanet picked up some rumors and refuted them for the first time to set the record straight.

　　1. [Rumor] Lanzhou Lamian Noodles can’t be eaten? All Lanzhou ramen noodles are using ramen. The main ingredient of ramen is penghui, a chemical substance containing a large amount of carcinogen-arsenic. Now all Lanzhou Lamian Noodles use this preparation to make flour more elastic. If the putty is put into a paper cup, it will be oxidized, and if the ramen solution is poured on the smooth floor, it will be even more horrible.

　　[Rumor] This rumor appeared in 2013. At that time, the media had rumored that the traditional technology of Lanzhou beef noodles pays attention to "three times of water, three times of ash, and 9981 times of kneading". Among them, ash refers to Peng ash, which can make the pulled noodles smooth and yellow and strong. Later, the process was replaced by a ramen agent, which is to purify the ash and get rid of harmful substances. At present, the Lanzhou market uses the ramen agent developed by Lanzhou University, Jiaotong University and related enterprises, and has passed the relevant safety certification.

　　2, [rumor] Casserole porridge is also "added", which will cause cancer? There are three shady things about online casserole porridge: 1. Adding synthetic essence; 2 add red shrimp powder; 3 add dead shrimps and crabs. Almost every family must add spices, vanillin and ethyl maltol, if eaten in excess, liver and kidney will be damaged, which will seriously cause cancer!

　　[Rumor] It is understood that in order to improve the taste of porridge foods or keep them in a good viscous state, food producers and operators usually use thickeners in porridge foods. Thickener is a kind of food additive with high safety, such as xanthan gum, edible alkali and phosphate. Experts believe that as long as it is used according to the regulations, it will not cause harm to the human body, but it should follow the principle of reducing the usage as much as possible under the premise of achieving the expected effect.

　　Rumor link: porridge shop thick rice porridge is "thickening porridge"? Investigation: No thickening agent is needed.

　　3, [rumor] exposure! Pork mixed with mouse meat

　　[Rumor] In the meat bowl that appeared in the article, it was said that the restaurant used mouse meat. In fact, netizens mistook the game returned from their homes for the restaurant cooking with mouse meat.

　　4. [Proverbs] Egg yolk pie and durian crisp are junk food in junk food.

　　[Rumor] It is almost impossible to avoid eating trans fatty acids in daily life, because they are found in cattle and sheep products and dairy products. In 2013, the report "China Residents’ Dietary Intake Level of Trans Fatty Acids and Its Risk Assessment" released by the National Food Safety Risk Assessment Center showed that the average trans fatty acid consumption in Chinese was 0.39 grams per day, and the energy provided by trans fatty acids through dietary intake accounted for 0.16% of the total dietary energy, which was far lower than the recommended value of the World Health Organization (less than 1%). So as long as you control the intake of trans fatty acids, you don’t need to be too anxious.

　　Rumor link: trans fatty acid risk is exaggerated? Expert: Risk control should be treated scientifically.

　　5. [Proverbs] Don’t drink unified milk tea or other milk-containing beverages (unified milk tea, Assam milk tea, Earl milk tea, Yaha coffee). The so-called milk component is non-dairy creamer, with a low milk content, and the main component is hydrogenated vegetable oil.

　　[Rumor] The ingredient list of unified Assam milk tea does indicate that it contains hydrogenated vegetable oil, but it also indicates that it contains skim milk powder. There is no situation of deceiving consumers that milk is contained in products but other food additives are used as milk powder. The concern about hydrogenated vegetable oil and non-dairy creamer actually lies in the trans fatty acids contained in them. Xinhuanet has made a special rumor about this. Experts said that the energy supply ratio of trans fatty acids in China is low, and consumers do not need to talk about "acid" discoloration.

　　Rumor link: 2017 trans fatty acid press conference

　　6, [rumor] net biography "The snake fruit is all wax when scraped, and it can be lit with a lighter!

　　[Rumor] In China, waxing is allowed on the surface of fruits. According to the Hygienic Standard for the Use of Food Additives, morpholine fatty acid salt (also known as fruit wax) and carnauba wax can be used as coating agents on the surface of fresh fruits. Fruit wax is a kind of chitosan substance, which is mostly extracted from crustaceans such as crabs and shells. This substance is harmless to the body after long-term consumption, and its function is mainly to keep fresh and prevent apples from rotting and deteriorating during long-distance transportation and long-term storage. If you are worried about health problems, you can peel apples and eat them. If you don’t want to peel them, you can rinse them with hot water at a suitable temperature to remove them.

　　7. [Proverbs] Drinking Coke every day = chronic suicide. Drinking Coke regularly on the Internet can cause hypokalemia in the body, showing some symptoms such as physical weakness, constipation and paralysis. People who drink 2 to 10 liters of cola every day will have symptoms of hypokalemia, which will lead to a series of diseases such as muscle disease, arrhythmia and paralysis.

　　[Rumor] Drink 2 to 10 liters of cola every day? How many people can drink such a "massive" coke every day? Moreover, rumors have seriously exaggerated the effect of cola on human body. Cola is a high-sugar beverage, and drinking it in large quantities is indeed harmful to certain people such as hyperglycemia and hyperlipidemia, but it will not become the main murderer of "chronic suicide".

　　8. [Rumor] The squab eaten may be a mouse. A group of pictures and videos of "See how unscrupulous merchants turn mice into squab" went viral on Weibo. Many netizens expressed shock and disgust at this. "I dare not eat squab indiscriminately in the future." Even a few netizens who doubted the authenticity of the video said that "there is a psychological shadow."

　　[Rumor] According to local reporters’ investigation, the mice in this group of photos were originally edible. The dishes in the pictures were "pepper and pepper rats", which were artificially bred and used for food, instead of the street rats as rumored. Mountain hole mouse, the scientific name of bamboo mouse, also known as bamboo distillation, mang raccoon, bamboo raccoon, bamboo root mouse, winter hairy mouse, etc., belongs to the genus Bambusoideae, a mammal rodent. It is big and fleshy, delicious, nutritious, thick and soft, and has high economic value.

　　9. [Proverbs] Have you ever seen such an egg? Egg white, egg yolk and eggshell are all made of chemical raw materials, and there is no natural ingredient! Recently, some people in the night market food stalls in Zhengzhou are buying this kind of "pure artificial egg". Mr. Wang, who deals in food additives, found this kind of egg, and asked the stall owner to make it. When he went home to try it, he could really make it into a "pure artificial egg".

　　Weibo @ Jiangning Public Security Online, the official of Jiangning Branch of Nanjing Public Security Bureau, said that the video was a process of making toy eggs. Don’t worry about it. The media reporter found that this kind of toy egg can indeed be found on the Internet. The yellow label on it is exactly the same as that in the video. Fake egg, really not. The main thing is that eggshells can’t be made. Besides, eggs are so cheap, even if the shell-free fake egg can be made, the cost is much higher than that of real eggs.

　　Rumor link: Top Ten Rumors in April: Did fake egg pour cucumber into the market because of spraying drugs?

　　10. [Proverbs] Cucumber coated with birth control pills will be sterilized after eating.

　　[Rumor] "Contraceptive cucumber" is purely a rumor. People often say that birth control pills are animal hormones, which have no effect on the growth and development of plant cucumbers and cannot be used on cucumbers. People are rumored to be birth control pills, probably because the plant growth regulator used on cucumber can promote the growth and development of cucumber and has the function of hormone, and birth control pills are also hormones. As a result, it was passed around, and finally it was spread that cucumbers used contraceptives.

　　Rumor link: "Contraceptive cucumber" is true? Expert: It is purely a rumor.

　　11. [Proverbs] Something happened to the fruit orange! Coca-Cola admits that its fruit oranges contain carbendazim, a pesticide banned in the United States. Experts say that carbendazim can cause brain paralysis and liver tumors.

　　[Rumor] In mid-January, 2012, Coca-Cola found carbendazim, a fungicide sprayed by Brazilian fruit farmers on fruit trees, and then reported it to the FDA of the US Food and Drug Administration. However, after the products were submitted for inspection by the United States and China respectively, the results showed that there was no problem. Carbendazim is allowed to be used in the EU and Brazil, the raw material of orange juice. The EU limits it to 200ppb, and the detected concentration is 35ppb, which is completely harmless to human body. The FDA finally concluded that "the detected carbendazim residue level is far below any standard that may pose a safety hazard, so consumers do not need to worry about orange juice products on the market."

　　The public can check related rumors, rumors and popular science information in the "China Food Rumor Alliance official website"

　　[Short Comment] It is urgent to crack down on food rumors.

　　In 2018, food rumors are still banned repeatedly, which makes us deeply feel the importance of controlling rumors. Experts have long pointed out that food rumors are rampant, to a large extent, because rumors and rumourers use potential food safety risks to deliberately fabricate, steal concepts, and even deliberately discredit them, inducing consumers to have distrust of them, and some consumers often lack scientific judgment.

　　"Eye game" has become the biggest motivation in the spread of rumors. In order to pursue "100,000+",some online media, especially self-media, play the role of "infected people" and help rumors spread like "epidemic viruses". Rumors of food safety have seriously interfered with the normal food and drug supervision, disturbed people’s consumption judgment, damaged the development of the industry and affected China’s international reputation.

　　To control food rumors, we must make a heavy blow and promote the establishment of a long-term mechanism for social multi-subjects to jointly control rumors. China food and drug administration and the "China Food Rumor Alliance" have spared no effort to carry out rumors and control rumors. In 2017, the State Food and Drug Administration and 10 departments jointly issued the Notice on Strengthening the Prevention and Control of Food Safety Rumors, and at the same time actively released regulatory information on multiple platforms, and established a rumor database and a popular science network platform. Since last year, the General Administration has published 85 rumors, and the database has now included more than 30,000 rumors. In addition, the State Food and Drug Administration also cooperated with the public security and online information departments to carry out joint law enforcement and detain and punish the producers of food rumors.

　　Up to now, China Food Rumor Alliance has released more than 70 authoritative rumors, and published and forwarded 1,000 related food popular science information, which has made an important contribution to guiding the public out of the misunderstanding of food safety. At the same time, the alliance also calls for the establishment of a cooperative mechanism, and at the same time, we should continue to strengthen the punishment and accountability of those who make and spread rumors, especially to clarify the rules for judging rumors and the quantitative standards for harm, and further strengthen the crackdown on rumors related to food networks by laws and regulations such as the Criminal Law and the Food Safety Law, so as to provide a clear and harmonious network environment for China’s food industry and consumers. (Contributed by China Food Rumor Alliance)

On November 9, 2018, on the occasion of Tencent’s 20th birthday, Tencent announced a major event. The Tencent Foundation will invest 1 billion yuan to launch an award "Scientific Exploration Award" aimed at supporting young scientists, encouraging and funding young scientists to invest in basic science and cutting-edge technology research.

CCTV News:With the National Day approaching, Dounan Flower Market in Kunming, Yunnan Province has also ushered in a wave of consumption boom.As the largest fresh cut flower market in Asia, it is not only a "wind vane" but also a "barometer" for the sales of fresh cut flowers in Asia. The flowers shipped from here every day flow to more than 50 countries and regions around the world, decorating the lives of thousands of families.

In Dounan Flower Market, Jiao Xiuzhi, a tourist from Shanxi, is excitedly video-chatting with friends, visiting the flower market and recommending flowers from Yunnan to friends.

Due to the increase of happy events such as opening, housewarming and marriage during the National Day, flower varieties such as Phalaenopsis, roses and Eupatorium grandiflorum are also showing a booming trend of purchase and sale. Merchants prepared their goods early, and the roads were lined with flowers. Those brightly colored flowers were placed in the front row for customers to choose.

At the express delivery point, the couriers have already started busy packing. With the rapid development of logistics, more than half of Dounan’s flowers sent to the whole country or exported have been transported by air, and 80% of them have been transported by road with cold chain vehicles controlled by low temperature. While effectively reducing flower loss, the freshness and quality of flowers are guaranteed, thus attracting more potential consumers.

In a flower trading platform in Kunming, the staff are busy negotiating the transaction. This is the first vertical e-commerce service platform in China to match online transactions between flower merchants and flower farmers. There are more than 3,500 flower farmers and cooperatives, and more than 300,000 buyers have settled in. Up to now, the cumulative transaction volume of flowers has exceeded 1 billion, and the cumulative transaction volume has exceeded 1 billion yuan.